Visible light optical coherence tomography angiography (vis-OCTA) facilitates local microvascular oximetry in the human retina

WEIYE SONG, WENJUN SHAO, WEI YI, RONGRONG LIU, MANISHI DESAI, STEVEN NESS, JI YI

Research output: Contribution to journalArticlepeer-review

Abstract

We report herein the first visible light optical coherence tomography angiography (vis-OCTA) for human retinal imaging. Compared to the existing vis-OCT systems, we devised a spectrometer with a narrower bandwidth to increase the spectral power density for OCTA imaging, while retaining the major spectral contrast in the blood. We achieved a 100 kHz A-line rate, the fastest acquisition speed reported so far for human retinal vis-OCT. We rigorously optimized the imaging protocol such that a single acquisition took < 6 seconds with a field of view (FOV) of 3X7.8 mm2. The angiography enables accurate localization of microvasculature down to the capillary level and thus enables oximetry at vessels < 100 μm in diameter. We demonstrated microvascular hemoglobin oxygen saturation (sO2) at the feeding and draining vessels at the perifoveal region. The longitudinal repeatability was assessed by < 5% coefficient of variation (CV). The unique capabilities of our vis-OCTA system may allow studies on the role of microvascular oxygen in various retinal pathologies.

Original languageEnglish (US)
Pages (from-to)4037-4051
Number of pages15
JournalBiomedical Optics Express
Volume11
Issue number7
DOIs
StatePublished - Jul 1 2020
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

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