Visible-light optical coherence tomography angiography for monitoring laser-induced choroidal neovascularization in mice

Ronil S. Shah, Brian T. Soetikno, Ji Yi, Wenzhong Liu, Dimitra Skondra, Hao F. Zhang, Amani A. Fawzi

Research output: Contribution to journalArticlepeer-review

Abstract

PURPOSE. This study sought to determine the earliest time-point at which evidence of choroidal neovascularization (CNV) could be detected with visible-light optical coherence tomography angiography (vis-OCTA) in a mouse model of laser-induced CNV. METHODS. Visible light-OCTA was used to study laser-induced CNVat different time-points after laser injury to monitor CNV development and measure CNV lesion size. Measurements obtained from vis-OCTA angiograms were compared with histopathologic measurements from isolectin-stained choroidal flatmounts. RESULTS. Choroidal neovascularization area measurements between the vis-OCTA system and isolectin-stained choroidal flatmounts were significantly different in area for days 2 to 4 postlaser injury, and were not significantly different in area for days 5, 7, and 14. Choroidal neovascularization area measurements taken from the stained flatmounts were larger than their vis-OCTA counterparts for all time-points. Both modalities showed a similar trend of CNV size increasing from the day of laser injury until a peak of day 7 postlaser injury and subsequently decreasing by day 14. CONCLUSIONS. The earliest vis-OCTA can detect the presence of aberrant vessels in a mouse laser-induced CNV model is 5 days after laser injury. Visible light-OCTA was able to visualize the maximum of the CNV network 7 days postlaser injury, in accordance with choroidal flatmount immunostaining. Visible light-OCTA is a reliable tool in both detecting the presence of CNV development, as well as accurately determining the size of the lesion in a mouse laserinduced CNV model.

Original languageEnglish (US)
Pages (from-to)OCT86-OCT95
JournalInvestigative Ophthalmology and Visual Science
Volume57
Issue number9
DOIs
StatePublished - Jul 2016
Externally publishedYes

Keywords

  • Age-related macular degeneration
  • Laser-induced choroidal neovascularization
  • Optical coherence tomography

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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