TY - JOUR
T1 - Virus isolations from mosquitoes collected during the 1982 Japanese encephalitis epidemic in Northern Thailand
AU - Leake, C. J.
AU - Ussery, M. A.
AU - Nisalak, A.
AU - Hoke, C. H.
AU - Andre, R. G.
AU - Burke, D. S.
N1 - Funding Information:
We would like to thank Dr. Thanom Laorakpong-se, Head, KamphaengphePt rovincial Hospital, for his support throughoutt his study. Our thanks are alsod ue to the skilledt echnicianso f the Departments of Virology and Medical Entomologya t AFRIMS. We thank Air Vice Marshall Sitibun Purnaveja, Director AFRIMS, and Dr. M. Beneson,D irector U.S. Component, AFRIMS, for their continued support and encouragementD. r C. J. Leake was supportedb y a grant from the WellcomeT rust.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1986
Y1 - 1986
N2 - From 16 June to 15 August, 1982 CDC light traps were used to collect mosquitoes in the province of Kamphaengphet, N. Thailand. 353, 042 mosquitoes comprising 59 species were collected and identified, and 345, 173 were placed in pools for attempted virus isolation by inoculation of C6/36 Aedes albopictus mosquito cell cultures. Viruses were isolated from 63 mosquito pools. These comprised 56 flaviviruses, identified as 35 isolates of Japanese encephalitis (JE) virus strains, 18 strains of Tembusu (TEM) virus and three untyped flaviviruses (FLA); three alphaviruses, identified as the first isolates of Getah (GET) virus to have been made in Thailand; and four viruses which are still unidentified. Most virus isolates were from Culex tritaeniorhynchus mosquitoes collected in carbon dioxide baited light traps. JE virus was isolated only over a ten-day period and the last isolate was obtained one week before the peak of admission of human encephalitis cases at Kamphaengphet Provincial Hospital. Rapid screening of isolates grown on Ae. pseudoscutellaris (LSTM-AP-61) mosquito cells by indirect immunofluorescence using flavivirus group-specific and JE-specific monoclonal antibodies showed a high degree of correlation with plaque reduction neutralization tests. An antigen capture enzyme immunoassay (EIA) test successfully identified about 50% of the JE virus positive pools, but the method saved considerable processing time.
AB - From 16 June to 15 August, 1982 CDC light traps were used to collect mosquitoes in the province of Kamphaengphet, N. Thailand. 353, 042 mosquitoes comprising 59 species were collected and identified, and 345, 173 were placed in pools for attempted virus isolation by inoculation of C6/36 Aedes albopictus mosquito cell cultures. Viruses were isolated from 63 mosquito pools. These comprised 56 flaviviruses, identified as 35 isolates of Japanese encephalitis (JE) virus strains, 18 strains of Tembusu (TEM) virus and three untyped flaviviruses (FLA); three alphaviruses, identified as the first isolates of Getah (GET) virus to have been made in Thailand; and four viruses which are still unidentified. Most virus isolates were from Culex tritaeniorhynchus mosquitoes collected in carbon dioxide baited light traps. JE virus was isolated only over a ten-day period and the last isolate was obtained one week before the peak of admission of human encephalitis cases at Kamphaengphet Provincial Hospital. Rapid screening of isolates grown on Ae. pseudoscutellaris (LSTM-AP-61) mosquito cells by indirect immunofluorescence using flavivirus group-specific and JE-specific monoclonal antibodies showed a high degree of correlation with plaque reduction neutralization tests. An antigen capture enzyme immunoassay (EIA) test successfully identified about 50% of the JE virus positive pools, but the method saved considerable processing time.
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U2 - 10.1016/0035-9203(86)90397-4
DO - 10.1016/0035-9203(86)90397-4
M3 - Article
C2 - 2885948
AN - SCOPUS:0022830997
SN - 0035-9203
VL - 80
SP - 831
EP - 837
JO - Transactions of the Royal Society of Tropical Medicine and Hygiene
JF - Transactions of the Royal Society of Tropical Medicine and Hygiene
IS - 5
ER -