Virulence factor-dependent basolateral invasion of choroid plexus epithelial cells by pathogenic Escherichia coli in vitro

Rebekah Rose, Svenja Häuser, Carolin Stump-Guthier, Christel Weiss, Manfred Rohde, Kwang Sik Kim, Hiroshi Ishikawa, Horst Schroten, Christian Schwerk, Rüdiger Adam

Research output: Contribution to journalArticle

Abstract

Escherichia coli is the most common Gram-negative causative agent of neonatal meningitis and E. coli meningitis is associated with high morbidity and mortality. Previous research has been carried out with regard to the blood-brain barrier and thereby unveiled an assortment of virulence factors involved in E. coli meningitis. Little, however, is known about the role of the blood-cerebrospinal fluid (CSF) barrier (BCSFB), in spite of several studies suggesting that the choroid plexus (CP) is a possible entry point for E. coli into the CSF spaces. Here, we used a human CP papilloma (HIBCPP) cell line that was previously established as valid model for the study of the BCSFB. We show that E. coli invades HIBCPP cells in a polar fashion preferentially from the physiologically relevant basolateral side. Moreover, we demonstrate that deletion of outer membrane protein A, ibeA or neuDB genes results in decreased cell infection, while absence of fimH enhances invasion, although causing reduced adhesion to the apical side of HIBCPP cells. Our findings suggest that the BCSFB might constitute an entry point for E. coli into the central nervous system, and HIBCPP cells are a valuable tool for investigating E. coli entry of the BCSFB.

Original languageEnglish (US)
JournalFEMS Microbiology Letters
Volume365
Issue number24
DOIs
StatePublished - Dec 1 2018

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Choroid Plexus
Virulence Factors
Epithelial Cells
Escherichia coli
Escherichia coli Meningitis
Cerebrospinal Fluid
Choroid Plexus Papilloma
Blood-Brain Barrier
Meningitis
Central Nervous System
In Vitro Techniques
Morbidity
Cell Line
Mortality
Infection
Research
Genes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

Cite this

Virulence factor-dependent basolateral invasion of choroid plexus epithelial cells by pathogenic Escherichia coli in vitro. / Rose, Rebekah; Häuser, Svenja; Stump-Guthier, Carolin; Weiss, Christel; Rohde, Manfred; Kim, Kwang Sik; Ishikawa, Hiroshi; Schroten, Horst; Schwerk, Christian; Adam, Rüdiger.

In: FEMS Microbiology Letters, Vol. 365, No. 24, 01.12.2018.

Research output: Contribution to journalArticle

Rose, R, Häuser, S, Stump-Guthier, C, Weiss, C, Rohde, M, Kim, KS, Ishikawa, H, Schroten, H, Schwerk, C & Adam, R 2018, 'Virulence factor-dependent basolateral invasion of choroid plexus epithelial cells by pathogenic Escherichia coli in vitro', FEMS Microbiology Letters, vol. 365, no. 24. https://doi.org/10.1093/femsle/fny274
Rose, Rebekah ; Häuser, Svenja ; Stump-Guthier, Carolin ; Weiss, Christel ; Rohde, Manfred ; Kim, Kwang Sik ; Ishikawa, Hiroshi ; Schroten, Horst ; Schwerk, Christian ; Adam, Rüdiger. / Virulence factor-dependent basolateral invasion of choroid plexus epithelial cells by pathogenic Escherichia coli in vitro. In: FEMS Microbiology Letters. 2018 ; Vol. 365, No. 24.
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