Gastroenteritis de etiología vírica. Aplicación de un protocolo para el diagnóstico de astrovirus en casos de gastroenteritis infantil

Translated title of the contribution: Viral gastroenteritis. Application of a protocol for astrovirus detection in childhood gastroenteritis

Raúl Ortiz De Lejarazu Leonardo, Francisco Javier Luquero Alcalde, José María Eiros Bouza, José Javier Castrodeza Sanz, Alberto Tenorio Abreu, Sonia Tamames Gómez, Irene Gracia Ahufinger, Juan Ignacio Reguera Useros

Research output: Contribution to journalArticlepeer-review

Abstract

BACKGROUND AND OBJECTIVE: Diagnosis of viral gastroenteritis is an important subject in clinical virology which is mainly determined by the availability of reagents in laboratories, such as in the case of astrovirus. The aim of this study was to estimate the increase in the diagnostic performance achieved after the incorporation of astrovirus search in the diagnosis protocol of acute viral gastroenteritis. We also analyzed the trend of infections in other more commonly searched virus, such as rotavirus and enteric adenovirus. MATERIAL AND METHODS: Retrospective study during 20 years that included 12,980 stool samples processed for gastroenteritis virus diagnosis. Since 1997 an enzyme immunoassay for astrovirus has been applied to those samples that are negative for rotavirus and adenovirus. The study was divided in two periods (1986-1996 and 1997-2005, without and with astrovirus diagnosis) and the percentage of patients diagnosed in each period was compared. The trend of positive results as well as the percentage of positive results over all patients studied was modelled using the least squares method. RESTULTS: The percentages of positive patients for rotavirus, adenovirus and astrovirus were 10.3%, 2.3% and 6.0% respectively, and there were uncommon co-infections by rotavirus and adenovirus (0.2%). The protocol applied to the astrovirus diagnosis increased the diagnosis rate up to 16.8% of the studied cases. Significant statistical differences were observed between the 2 study periods. A quadratic growth was observed in the results of positive diagnosis of viral gastroenteritis during the study period. CONCLUSIONS: The search of astrovirus in gastroenteritis cases by a selective protocol increased the diagnostic performance of gastrointestinal virus by 6%. In view of these results, it would be useful to implement astrovirus diagnosis in faeces with liquid or semi-liquid consistency when rotavirus and adenovirus detection is negative.

Translated title of the contributionViral gastroenteritis. Application of a protocol for astrovirus detection in childhood gastroenteritis
Original languageSpanish
Pages (from-to)365-369
Number of pages5
JournalMedicina clínica
Volume128
Issue number10
DOIs
StatePublished - Mar 17 2007
Externally publishedYes

Keywords

  • Adenovirus
  • Astrovirus
  • Diagnosis
  • Gastroenteritis
  • Rotavirus
  • Trend

ASJC Scopus subject areas

  • Medicine(all)

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