Vimentin supports mitochondrial morphology and organization

Ho Lam Tang, Hong Lok Lung, Ka Chun Wu, Anh Huy Phan Le, Ho Man Tang, Ming Chiu Fung

Research output: Contribution to journalArticlepeer-review

Abstract

Vimentin is one of the intermediate filaments that functions in structural support, signal transduction and organelle positioning of a cell. In the present study, we report the contribution of vimentin in mitochondrial morphology and organization. Using subcellular fractionation, immunoprecipitation and fluorescence microscopy analyses, we found that vimentin was associated with mitochondria. Knockdown of vimentin resulted in mitochondrial fragmentation, swelling and disorganization. We further demonstrated that the vimentin cytoskeleton co-localized and interacted with mitochondria to a greater extent than other cytoskeletal components known to support mitochondria. Our results also suggest that vimentin could participate in the mitochondrial association of microtubules. As mitochondrial morphologies determine mitochondrial function, our findings revealed a potentially important relationship between the vimentin-based intermediate filaments and the regulation of mitochondria.

Original languageEnglish (US)
Pages (from-to)141-146
Number of pages6
JournalBiochemical Journal
Volume410
Issue number1
DOIs
StatePublished - Feb 15 2008
Externally publishedYes

Keywords

  • Cytoskeleton
  • Interaction
  • Intermediate filament
  • Microtubule
  • Mitochondrion
  • Vimentin

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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