Viewing the bilayer hydrocarbon core using neutron diffraction

Xue Han; Kalina Hristova

doi:10.1007/s00232-008-9151-3

Viewing the bilayer hydrocarbon core using neutron diffraction

Xue Han, Kalina Hristova

Whiting School of Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

Membrane proteins fold, assemble and function within their native fluid lipid environment. Structural studies of fluid lipid bilayers are thus critically important for understanding processes in membranes. Here, we propose a simple approach to visualize the hydrocarbon core using neutron diffraction and deuterated lipids that are commercially available. This method should have broad utility in structural studies of the bilayer response to protein insertion and folding in membranes.

Original language	English (US)
Pages (from-to)	123-131
Number of pages	9
Journal	Journal of Membrane Biology
Volume	227
Issue number	3
DOIs	https://doi.org/10.1007/s00232-008-9151-3
State	Published - Feb 2009

Keywords

Biophysical techniques in membrane research
Membrane biophysics
Membrane structure

ASJC Scopus subject areas

Biophysics
Physiology
Cell Biology

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10.1007/s00232-008-9151-3

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title = "Viewing the bilayer hydrocarbon core using neutron diffraction",

abstract = "Membrane proteins fold, assemble and function within their native fluid lipid environment. Structural studies of fluid lipid bilayers are thus critically important for understanding processes in membranes. Here, we propose a simple approach to visualize the hydrocarbon core using neutron diffraction and deuterated lipids that are commercially available. This method should have broad utility in structural studies of the bilayer response to protein insertion and folding in membranes.",

keywords = "Biophysical techniques in membrane research, Membrane biophysics, Membrane structure",

author = "Xue Han and Kalina Hristova",

note = "Funding Information: We thank Drs. M. Mihailescu and D. Worcester for their help with neutron data collection and processing and for useful discussions. This work was supported by National Institutes of Health (NIH) grant GM068619 (to K. H.). We acknowledge the support of the National Institute of Standards and Technology, U.S. Department of Commerce, and the Cold Neutrons for Biology and Technology (CNBT) program funded by the NIH under grant RR14812 and the Regents of the University of California, for providing the neutron research facilities used in this work.",

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doi = "10.1007/s00232-008-9151-3",

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AU - Han, Xue

AU - Hristova, Kalina

N1 - Funding Information: We thank Drs. M. Mihailescu and D. Worcester for their help with neutron data collection and processing and for useful discussions. This work was supported by National Institutes of Health (NIH) grant GM068619 (to K. H.). We acknowledge the support of the National Institute of Standards and Technology, U.S. Department of Commerce, and the Cold Neutrons for Biology and Technology (CNBT) program funded by the NIH under grant RR14812 and the Regents of the University of California, for providing the neutron research facilities used in this work.

PY - 2009/2

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N2 - Membrane proteins fold, assemble and function within their native fluid lipid environment. Structural studies of fluid lipid bilayers are thus critically important for understanding processes in membranes. Here, we propose a simple approach to visualize the hydrocarbon core using neutron diffraction and deuterated lipids that are commercially available. This method should have broad utility in structural studies of the bilayer response to protein insertion and folding in membranes.

AB - Membrane proteins fold, assemble and function within their native fluid lipid environment. Structural studies of fluid lipid bilayers are thus critically important for understanding processes in membranes. Here, we propose a simple approach to visualize the hydrocarbon core using neutron diffraction and deuterated lipids that are commercially available. This method should have broad utility in structural studies of the bilayer response to protein insertion and folding in membranes.

KW - Biophysical techniques in membrane research

KW - Membrane biophysics

KW - Membrane structure

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Viewing the bilayer hydrocarbon core using neutron diffraction

Abstract

Keywords

ASJC Scopus subject areas

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