Vibrissa-based object localization in head-fixed mice

Daniel H. O'Connor, Nathan G. Clack, Daniel Huber, Takaki Komiyama, Eugene W. Myers, Karel Svoboda

Research output: Contribution to journalArticle

Abstract

Linking activity in specific cell types with perception, cognition, and action, requires quantitative behavioral experiments in genetic model systems such as the mouse. In head-fixed primates, the combination of precise stimulus control, monitoring of motor output, and physiological recordings over large numbers of trials are the foundation on which many conceptually rich and quantitative studies have been built. Choice-based, quantitative behavioral paradigms for head-fixed mice have not been described previously. Here, we report a somatosensory absolute object localization task for head-fixed mice. Mice actively used their mystacial vibrissae (whiskers) to sense the location of a vertical pole presented to one side of the head and reported with licking whether the pole was in a target (go) or a distracter (no-go) location. Mice performed hundreds of trials with high performance (>90% correct) and localized to <0.95 mm (<6° of azimuthal angle). Learning occurred over 1-2 weeks and was observed both within and across sessions. Mice could perform object localization with single whiskers. Silencing barrel cortex abolished performance to chance levels.Wemeasured whisker movement and shape for thousands of trials. Mice moved their whiskers in a highly directed, asymmetric manner, focusing on the target location. Translation of the base of the whiskers along the face contributed substantially to whisker movements. Mice tended to maximize contact with the go (rewarded) stimulus while minimizing contact with the no-go stimulus.Weconjecture that this may amplify differences in evoked neural activity between trial types.

Original languageEnglish (US)
Pages (from-to)1947-1967
Number of pages21
JournalJournal of Neuroscience
Volume30
Issue number5
DOIs
StatePublished - Feb 3 2010

ASJC Scopus subject areas

  • Neuroscience(all)

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    O'Connor, D. H., Clack, N. G., Huber, D., Komiyama, T., Myers, E. W., & Svoboda, K. (2010). Vibrissa-based object localization in head-fixed mice. Journal of Neuroscience, 30(5), 1947-1967. https://doi.org/10.1523/JNEUROSCI.3762-09.2010