VI effects markedly attenuate V2 effects of AVP on urea transporter (UT) mRNA level in thin descending limb and initial inner medullary collecting duct

M. M. Trinh-Trang-Tan, D. Promeneur, L. Bankir

Research output: Contribution to journalArticle

Abstract

The mRNA level of short (S-) and long (L-) UT2 transcripts (tubular), and that of UT11 (vascular) were measured in Brattleboro rats with diabetes insipidus (DI), treated for 5 days with either AVP (5 μg/day) or the V2 agonist dDAVP (0.25 μg/day), and in control (Co) DI rats. Specific mRNAs were quantified by Northern analysis of 7 μg total RNA extracted from superficial and deep inner stripe (s-IS and d-IS) of outer medulla, and from base and tip of inner medulla (IM). mRNA levels are expressed as arbitrary units of density. Results are means ± SEM (n =3 ). AVP and dDAVP induced similar Uosm (2167 ± 79 for AVP, and 1831 ± 106 for dDAVP, vs 220 ± 45 mOsm / kg H2O for Co). Both hormones induced similar decrease in UT11 in whole IM, and similar increase in S-UT2 in s- and d-IS. Long UT2 of tip-IM was unchanged. However AVP induced much less increase in the following parameters than did dDAVP: S-UT2 in d-IS S-UT2 in base IM L-UT2 in base IM Co Exp Co Exp Co Exp AVP: 91 ± 21 247 ± 22* 21 ± 2 185 ± 38*65 ± 11 108 ± 33 dDAVP: 183 ± 9 # 2093 ± 130*# 19 ± 5 1139 ± 148*# 42 ± 8 803 ± 11*#*: p <0.05 or less vs Co; #: p <0.05 or less vs AVP (Student's t test) AVP and dDAVP induce similar changes in UT11 mRNA expression. In contrast, the attenuated effect of AVP vs dDAVP on expression of UT in thin descending limb (S-UT2), and in initial IM collecting duct (L-UT2 in base IM), likely results from blunted V2 effect by V1 effect of AVP.

Original languageEnglish (US)
JournalFASEB Journal
Volume11
Issue number3
StatePublished - 1997
Externally publishedYes

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diabetes insipidus
Ducts
transporters
Extremities
urea
Messenger RNA
Diabetes Insipidus
rats
blood vessels
agonists
hormones
Brattleboro Rats
RNA
Medical problems
Rats
Blood Vessels
Hormones
Students
urea transporter
Sand

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

VI effects markedly attenuate V2 effects of AVP on urea transporter (UT) mRNA level in thin descending limb and initial inner medullary collecting duct. / Trinh-Trang-Tan, M. M.; Promeneur, D.; Bankir, L.

In: FASEB Journal, Vol. 11, No. 3, 1997.

Research output: Contribution to journalArticle

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abstract = "The mRNA level of short (S-) and long (L-) UT2 transcripts (tubular), and that of UT11 (vascular) were measured in Brattleboro rats with diabetes insipidus (DI), treated for 5 days with either AVP (5 μg/day) or the V2 agonist dDAVP (0.25 μg/day), and in control (Co) DI rats. Specific mRNAs were quantified by Northern analysis of 7 μg total RNA extracted from superficial and deep inner stripe (s-IS and d-IS) of outer medulla, and from base and tip of inner medulla (IM). mRNA levels are expressed as arbitrary units of density. Results are means ± SEM (n =3 ). AVP and dDAVP induced similar Uosm (2167 ± 79 for AVP, and 1831 ± 106 for dDAVP, vs 220 ± 45 mOsm / kg H2O for Co). Both hormones induced similar decrease in UT11 in whole IM, and similar increase in S-UT2 in s- and d-IS. Long UT2 of tip-IM was unchanged. However AVP induced much less increase in the following parameters than did dDAVP: S-UT2 in d-IS S-UT2 in base IM L-UT2 in base IM Co Exp Co Exp Co Exp AVP: 91 ± 21 247 ± 22* 21 ± 2 185 ± 38*65 ± 11 108 ± 33 dDAVP: 183 ± 9 # 2093 ± 130*# 19 ± 5 1139 ± 148*# 42 ± 8 803 ± 11*#*: p <0.05 or less vs Co; #: p <0.05 or less vs AVP (Student's t test) AVP and dDAVP induce similar changes in UT11 mRNA expression. In contrast, the attenuated effect of AVP vs dDAVP on expression of UT in thin descending limb (S-UT2), and in initial IM collecting duct (L-UT2 in base IM), likely results from blunted V2 effect by V1 effect of AVP.",
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