Vesicle trafficking and cell surface membrane patchiness

Qing Tang, Michael A Edidin

Research output: Contribution to journalArticle

Abstract

Membrane proteins and lipids often appear to be distributed in patches on the cell surface. These patches are often assumed to be membrane domains, arising from specific molecular associations. However, a computer simulation (Gheber and Edidin, 1999) shows that membrane patchiness may result from a combination of vesicle trafficking and dynamic barriers to lateral mobility. The simulation predicts that the steady-state patches of proteins and lipids seen on the cell surface will decay if vesicle trafficking is inhibited. To test this prediction, we compared the apparent sizes and intensities of patches of class I HLA molecules, integral membrane proteins, before and after inhibiting endocytic vesicle traffic from the cell surface, either by incubation in hypertonic medium or by expression of a dominant-negative mutant dynamin. As predicted by the simulation, the apparent sizes of HLA patches increased, whereas their intensities decreased after endocytosis and vesicle trafficking were inhibited.

Original languageEnglish (US)
Pages (from-to)196-203
Number of pages8
JournalBiophysical Journal
Volume81
Issue number1
StatePublished - 2001

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Cell Membrane
Membrane Proteins
Dynamins
Transport Vesicles
Membranes
Membrane Lipids
Endocytosis
Computer Simulation
Lipids
Proteins

ASJC Scopus subject areas

  • Biophysics

Cite this

Vesicle trafficking and cell surface membrane patchiness. / Tang, Qing; Edidin, Michael A.

In: Biophysical Journal, Vol. 81, No. 1, 2001, p. 196-203.

Research output: Contribution to journalArticle

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