TY - JOUR
T1 - Vertical Transmission of Zika Virus (Flaviviridae, Flavivirus) in Amazonian Aedes aegypti (Diptera
T2 - Culicidae) Delays Egg Hatching and Larval Development of Progeny
AU - Chaves, Barbara Aparecida
AU - Junior, Ademir Bentes Vieira
AU - Silveira, Karine Renata Dias
AU - Paz, Andreia Da Costa
AU - Vaz, Evelyn Beatriz Da Costa
AU - Araujo, Raphaela Guedes Pereira
AU - Rodrigues, Nilton Barnabe
AU - Campolina, Thais Bonifacio
AU - Orfano, Alessandra Da Silva
AU - Nacif-Pimenta, Rafael
AU - Villegas, Luis Eduardo Martinez
AU - Melo, Fabrício Freire De
AU - Silva, Breno De Mello
AU - Monteiro, Wuelton Marcelo
AU - Guerra, Maria Das Graças Vale Barbosa
AU - Lacerda, Marcus Vinicius Guimarães De
AU - Norris, Douglas Eric
AU - Secundino, Nagila Francinete Costa
AU - Pimenta, Paulo Filemon Paolucci
N1 - Funding Information:
This study was partially funded by the following Brazilian agencies: Foundation of the Institute Oswaldo Cruz (FIOCRUZ), Strategic Program for Supporting Health Research (PAPES VI), Brazilian Council for Scientific and Technological Development (CNPq/MS/CAPES/DECIT), Program Science without Borders, INCT-Instituto Nacional de Ciencia e Tecnologia -Entomologia Molecular, Minas Gerais State Research Support Foundation (FAPEMIG), and Amazonas State Research Support Foundation (FAPEAM). Support Program to Young Ph.D. Professors – Federal University of Bahia (PROPESQ/UFBA). N.F.C.S., M.V.G.L., M.G.V.B.G., and P.F.P.P. are research fellows, supported by CNPq. B.A.C., T.B.C., A.S.O., E.B.C.V., and N.B.R. are student fellows supported by CAPES and K.R.D.S., L.E.M.V., and R.N.P. are supported by CNPq; A.C.P. and A.B.V.J. are supported by FAPEAM.
Publisher Copyright:
© 2019 The Author(s). Published by Oxford University Press on behalf of Entomological Society of America.
PY - 2019/10/28
Y1 - 2019/10/28
N2 - Zika virus (ZIKV) has emerged as a globally important arbovirus and has been reported from all states of Brazil. The virus is primarily transmitted to humans through the bite of an infective Aedes aegypti (Linnaeus, 1762) or Aedes albopictus (Skuse, 1895). However, it is important to know if ZIKV transmission also occurs from Ae. aegypti through infected eggs to her offspring. Therefore, a ZIKV and dengue virus (DENV) free colony was established from eggs collected in Manaus and maintained until the third-fourth generation in order to conduct ZIKV vertical transmission (VT) experiments which used an infectious bloodmeal as the route of virus exposure. The eggs from ZIKV-infected females were allowed to hatch. The resulting F1 progeny (larvae, pupae, and adults) were quantitative polymerase chain reaction (qPCR) assayed for ZIKV. The viability of ZIKV vertically transmitted to F1 progeny was evaluated by cultivation in C6/36 cells. The effects of ZIKV on immature development of Ae. aegypti was assessed and compared with noninfected mosquitoes. AmazonianAe. aegypti were highly susceptible to ZIKV infection (96.7%), and viable virus passed to their progeny via VT. Moreover, eggs from the ZIKV-infected mosquitoes had a significantly lower hatch rate and the slowest hatching. In addition, the larval development period was slower when compared to noninfected, control mosquitoes. This is the first study to illustrate VT initiated by oral infection of the parental population by using mosquitoes, which originated from the field and a ZIKV strain that is naturally circulating in-country. Additionally, this study suggests that ZIKV present in the Ae. aegypti can modify the mosquito life cycle. The data reported here suggest that VT of ZIKV to progeny from naturally infected females may have a critical epidemiological role in the dissemination and maintenance of the virus circulating in the vector.
AB - Zika virus (ZIKV) has emerged as a globally important arbovirus and has been reported from all states of Brazil. The virus is primarily transmitted to humans through the bite of an infective Aedes aegypti (Linnaeus, 1762) or Aedes albopictus (Skuse, 1895). However, it is important to know if ZIKV transmission also occurs from Ae. aegypti through infected eggs to her offspring. Therefore, a ZIKV and dengue virus (DENV) free colony was established from eggs collected in Manaus and maintained until the third-fourth generation in order to conduct ZIKV vertical transmission (VT) experiments which used an infectious bloodmeal as the route of virus exposure. The eggs from ZIKV-infected females were allowed to hatch. The resulting F1 progeny (larvae, pupae, and adults) were quantitative polymerase chain reaction (qPCR) assayed for ZIKV. The viability of ZIKV vertically transmitted to F1 progeny was evaluated by cultivation in C6/36 cells. The effects of ZIKV on immature development of Ae. aegypti was assessed and compared with noninfected mosquitoes. AmazonianAe. aegypti were highly susceptible to ZIKV infection (96.7%), and viable virus passed to their progeny via VT. Moreover, eggs from the ZIKV-infected mosquitoes had a significantly lower hatch rate and the slowest hatching. In addition, the larval development period was slower when compared to noninfected, control mosquitoes. This is the first study to illustrate VT initiated by oral infection of the parental population by using mosquitoes, which originated from the field and a ZIKV strain that is naturally circulating in-country. Additionally, this study suggests that ZIKV present in the Ae. aegypti can modify the mosquito life cycle. The data reported here suggest that VT of ZIKV to progeny from naturally infected females may have a critical epidemiological role in the dissemination and maintenance of the virus circulating in the vector.
KW - Aedes aegypti
KW - Zika virus
KW - fitness cost
KW - vertical transmission
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U2 - 10.1093/jme/tjz110
DO - 10.1093/jme/tjz110
M3 - Article
C2 - 31278737
AN - SCOPUS:85074306112
VL - 56
SP - 1739
EP - 1744
JO - Journal of Medical Entomology
JF - Journal of Medical Entomology
SN - 0022-2585
IS - 6
ER -