Verapamil blocks basal and angiotensin II-induced RNA synthesis of rat aortic vascular smooth muscle cells

Nabil S. Andrawis, Darrell R. Abernethy

Research output: Contribution to journalArticle

Abstract

We evaluated VER effect on RNA synthesis of quiescent and angiotensin II (AII)- stimulated cultured rat aortic vascular smooth muscle cells (VSMC). In a dose-dependent manner, VER decreased [3H]uridine uptake by quiescent VSMCs (ED50 7×10-6M), an effect that was shared by other calcium antagonists, but to a variable degree. VER caused a significant effect within 3 hours and attained a maximal effect at 7 hours. In addition VER caused a 22±2% decrease in [3H]uridine uptake by VSMCs stimulated with 10% fetal bovine serum, while it completely abolished [3H]uridine uptake by VSMCs induced by AII. We conclude that VER decreases basal and inhibits AII-induced increase in mRNA synthesis of VSMCs. These data may explain in part how VER causes a decrease in vascular resistance and alters the vasoconstrictor effect of AII.

Original languageEnglish (US)
Pages (from-to)767-773
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume183
Issue number2
DOIs
StatePublished - Mar 16 1992

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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