Abstract
A sensitive (to 0.5 ng/ml) and specific method for the determination of verapamil and norverapamil which utilizes gas-liquid chromatography with nitrogen-phosphorus detection is described. A basic extraction with acid back-wash and final basic reextraction is used for the preparation of plasma samples. Standard curves using α-isopropyl-α-[(N-methyl-N-homoveratryl)-β-aminoethyl]-3,4-dimethoxyphe nylacetonitrile hydrochloride (D-517) are linear for concentrations from 0.5 to 200 ng/ml for both verapamil and norverapamil. Within-day and between-day reproducibility is good with a coefficient of variation less than 10% for all concentrations. Recovery is complete for both verapamil and norverapamil. Applicability of the method is demonstrated by a pharmacokinetic study in a normal volunteer who received 10 mg verapamil hydrochloride by intravenous infusion.
Original language | English (US) |
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Pages (from-to) | 264-268 |
Number of pages | 5 |
Journal | Pharmacology |
Volume | 29 |
Issue number | 5 |
DOIs | |
State | Published - Jan 1 1984 |
ASJC Scopus subject areas
- Pharmacology