Verapamil and norverapamil determination in human plasma by gas-liquid chromatography using nitrogen-phosphorus detection: Application to single-dose pharmacokinetic studies

D. R. Abernethy, E. L. Todd, J. R. Mitchell

Research output: Contribution to journalArticle


A sensitive (to 0.5 ng/ml) and specific method for the determination of verapamil and norverapamil which utilizes gas-liquid chromatography with nitrogen-phosphorus detection is described. A basic extraction with acid back-wash and final basic reextraction is used for the preparation of plasma samples. Standard curves using α-isopropyl-α-[(N-methyl-N-homoveratryl)-β-aminoethyl]-3,4-dimethoxyphe nylacetonitrile hydrochloride (D-517) are linear for concentrations from 0.5 to 200 ng/ml for both verapamil and norverapamil. Within-day and between-day reproducibility is good with a coefficient of variation less than 10% for all concentrations. Recovery is complete for both verapamil and norverapamil. Applicability of the method is demonstrated by a pharmacokinetic study in a normal volunteer who received 10 mg verapamil hydrochloride by intravenous infusion.

Original languageEnglish (US)
Pages (from-to)264-268
Number of pages5
Issue number5
Publication statusPublished - 1984
Externally publishedYes


ASJC Scopus subject areas

  • Pharmacology

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