Ventral midbrain astrocytes display unique physiological features and sensitivity to dopamine D2 receptor signaling

Wendy Xin, Kornel Schuebel, Kam wing Jair, Raffaello Cimbro, Lindsay M. De Biase, David Goldman, Antonello Bonci

Research output: Contribution to journalArticle

Abstract

Astrocytes are ubiquitous CNS cells that support tissue homeostasis through ion buffering, neurotransmitter recycling, and regulation of CNS vasculature. Yet, despite the essential functional roles they fill, very little is known about the physiology of astrocytes in the ventral midbrain, a region that houses dopamine-releasing neurons and is critical for reward learning and motivated behaviors. Here, using a combination of whole-transcriptome sequencing, histology, slice electrophysiology, and calcium imaging, we performed the first functional and molecular profiling of ventral midbrain astrocytes and observed numerous differences between these cells and their telencephalic counterparts, both in their gene expression profile and in their physiological properties. Ventral midbrain astrocytes have very low membrane resistance and inward-rectifying potassium channel-mediated current, and are extensively coupled to surrounding oligodendrocytes through gap junctions. They exhibit calcium responses to glutamate but are relatively insensitive to norepinephrine. In addition, their calcium activity can be dynamically modulated by dopamine D2 receptor signaling. Taken together, these data indicate that ventral midbrain astrocytes are physiologically distinct from astrocytes in cortex and hippocampus. This work provides new insights into the extent of functional astrocyte heterogeneity within the adult brain and establishes the foundation for examining the impact of regional astrocyte differences on dopamine neuron function and susceptibility to degeneration.

Original languageEnglish (US)
JournalNeuropsychopharmacology
DOIs
StateAccepted/In press - Jan 1 2018
Externally publishedYes

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Dopamine D2 Receptors
Mesencephalon
Astrocytes
Dopaminergic Neurons
Calcium
Transcriptome
Telencephalon
Electrophysiology
Gap Junctions
Potassium Channels
Oligodendroglia
Recycling
Reward
Neurotransmitter Agents
Glutamic Acid
Hippocampus
Histology
Norepinephrine
Homeostasis
Learning

ASJC Scopus subject areas

  • Pharmacology
  • Psychiatry and Mental health

Cite this

Ventral midbrain astrocytes display unique physiological features and sensitivity to dopamine D2 receptor signaling. / Xin, Wendy; Schuebel, Kornel; Jair, Kam wing; Cimbro, Raffaello; De Biase, Lindsay M.; Goldman, David; Bonci, Antonello.

In: Neuropsychopharmacology, 01.01.2018.

Research output: Contribution to journalArticle

Xin, Wendy ; Schuebel, Kornel ; Jair, Kam wing ; Cimbro, Raffaello ; De Biase, Lindsay M. ; Goldman, David ; Bonci, Antonello. / Ventral midbrain astrocytes display unique physiological features and sensitivity to dopamine D2 receptor signaling. In: Neuropsychopharmacology. 2018.
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