VCaP, a cell-based model system of human prostate cancer

S. Korenchuk, J. E. Lehr, L. McLean, Y. G. Lee, S. Whitney, R. Vessella, D. L. Lin, Kenneth Pienta

Research output: Contribution to journalArticle

Abstract

Objectives: We report the isolation and characterization of a novel prostate cancer cell line derived from a vertebral metastatic lesion, Vertebral-Cancer of the Prostate (VCaP). Methods: Prostate cancer tissue was harvested at autopsy from a metastatic lesion to a lumbar vertebral body of a patient with hormone refractory prostate cancer. This tissue was aseptically xenografted into SCID mice and later harvested and plated on tissue culture dishes. For characterization, soft agar clonegenic assay, in vivo xenograft growth, in vitro doubling time, karyotype analysis, immunocytochemistry for cytokeratin-18 expression immunochemistry for PSA (prostate specific antigen), RT PCR for PAP (prostatic acid phosphatase) and northern blot and western blot analysis to determine expression of Rb and p53, were performed. Androgen receptor expression was measured by transient transfection with a luciferase reporter construct. Results: VCaP cells are immortal in vitro and can be passaged serially in vivo. They express large quantities of prostate specific antigen (PSA). This cell line also expresses prostatic acid phosphatase (PAP), cytokeratin-18 and the androgen receptor, and is androgen sensitive in vitro and in vivo. Conclusions: This cell line was derived from a metastatic tumor to the vertebrae of a prostate cancer patient. It exhibits many of the characteristics of clinical prostate carcinoma, including expression of PSA, PAP, and AR. We believe that VCaP will be a useful addition to the existing models of prostate cancer, and enable more advanced study of the mechanisms of prostate cancer progression and metastasis.

Original languageEnglish (US)
Pages (from-to)163-168
Number of pages6
JournalIn vivo (Athens, Greece)
Volume15
Issue number2
StatePublished - 2001
Externally publishedYes

Fingerprint

Prostate-Specific Antigen
Keratin-18
Prostatic Neoplasms
Cells
Androgen Receptors
Tissue
Tissue culture
Luciferases
Heterografts
Refractory materials
Androgens
Agar
Tumors
Assays
Hormones
Cell Line
Immunochemistry
prostatic acid phosphatase
SCID Mice
Karyotype

Keywords

  • Cell line
  • Model
  • Prostate cancer
  • Prostate specific antigen

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Korenchuk, S., Lehr, J. E., McLean, L., Lee, Y. G., Whitney, S., Vessella, R., ... Pienta, K. (2001). VCaP, a cell-based model system of human prostate cancer. In vivo (Athens, Greece), 15(2), 163-168.

VCaP, a cell-based model system of human prostate cancer. / Korenchuk, S.; Lehr, J. E.; McLean, L.; Lee, Y. G.; Whitney, S.; Vessella, R.; Lin, D. L.; Pienta, Kenneth.

In: In vivo (Athens, Greece), Vol. 15, No. 2, 2001, p. 163-168.

Research output: Contribution to journalArticle

Korenchuk, S, Lehr, JE, McLean, L, Lee, YG, Whitney, S, Vessella, R, Lin, DL & Pienta, K 2001, 'VCaP, a cell-based model system of human prostate cancer', In vivo (Athens, Greece), vol. 15, no. 2, pp. 163-168.
Korenchuk S, Lehr JE, McLean L, Lee YG, Whitney S, Vessella R et al. VCaP, a cell-based model system of human prostate cancer. In vivo (Athens, Greece). 2001;15(2):163-168.
Korenchuk, S. ; Lehr, J. E. ; McLean, L. ; Lee, Y. G. ; Whitney, S. ; Vessella, R. ; Lin, D. L. ; Pienta, Kenneth. / VCaP, a cell-based model system of human prostate cancer. In: In vivo (Athens, Greece). 2001 ; Vol. 15, No. 2. pp. 163-168.
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AU - Vessella, R.

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