VCAM1 is induced in ovarian theca and stromal cells in a mouse model of androgen excess

Nicholes R. Candelaria, Achuth Padmanabhan, Fabio Stossi, M. Cecilia Ljungberg, Katharine E. Shelly, Braden K. Pew, Minerva Solis, Ayane M. Rossano, Jan M. McAllister, Sheng Wu, Jo Anne S. Richards

Research output: Contribution to journalArticle

Abstract

Ovarian theca androgen production is regulated by the pituitary LH and intrafollicular factors. Enhanced androgen biosynthesis by theca cells contributes to polycystic ovary syndrome (PCOS) in women, but the ovarian consequences of elevated androgens are not completely understood. Our study documents the molecular events that are altered in the theca and stromal cells of mice exposed to high androgen levels, using the nonaromatizable androgen DHT. Changes in ovarian morphology and function were observed not only in follicles, but also in the stromal compartment. Genome-wide microarray analyses revealed marked changes in the ovarian transcriptome of DHT-treated females within 1 week. Particularly striking was the increased expression of vascular cell adhesion molecule 1 (Vcam1) specifically in the NR2F2/COUPTF-II lineage theca cells, not granulosa cells, of growing follicles and throughout the stroma of the androgen-treated mice. This response was mediated by androgen receptors (ARs) present in theca and stromal cells. Human theca-derived cultures expressed both ARs and NR2F2 that were nuclear. VCAM1 mRNA and protein were higher in PCOS-derived theca cells compared with control theca and reduced markedly by the AR antagonist flutamide. In the DHT-treated mice, VCAM1 was transiently induced by equine chorionic gonadotropin, when androgen and estrogen biosynthesis peak in preovulatory follicles, and was potently suppressed by a superovulatory dose of human chorionic gonadotropin. High levels of VCAM1 in the theca and interstitial cells of DHT-treated mice and in adult Leydig cells indicate that there may be novel functions for VCAM1 in reproductive tissues, including the gonads.

Original languageEnglish (US)
Pages (from-to)1377-1393
Number of pages17
JournalEndocrinology
Volume160
Issue number6
DOIs
StatePublished - Jun 1 2019

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Theca Cells
Stromal Cells
Androgens
Polycystic Ovary Syndrome
Androgen Receptors
Chorionic Gonadotropin
Androgen Receptor Antagonists
Equine Gonadotropins
Flutamide
Vascular Cell Adhesion Molecule-1
Leydig Cells
Granulosa Cells
Gonads
Microarray Analysis
Transcriptome
Estrogens
Genome
Messenger RNA

ASJC Scopus subject areas

  • Endocrinology

Cite this

Candelaria, N. R., Padmanabhan, A., Stossi, F., Cecilia Ljungberg, M., Shelly, K. E., Pew, B. K., ... Richards, J. A. S. (2019). VCAM1 is induced in ovarian theca and stromal cells in a mouse model of androgen excess. Endocrinology, 160(6), 1377-1393. https://doi.org/10.1210/en.2018-00731

VCAM1 is induced in ovarian theca and stromal cells in a mouse model of androgen excess. / Candelaria, Nicholes R.; Padmanabhan, Achuth; Stossi, Fabio; Cecilia Ljungberg, M.; Shelly, Katharine E.; Pew, Braden K.; Solis, Minerva; Rossano, Ayane M.; McAllister, Jan M.; Wu, Sheng; Richards, Jo Anne S.

In: Endocrinology, Vol. 160, No. 6, 01.06.2019, p. 1377-1393.

Research output: Contribution to journalArticle

Candelaria, NR, Padmanabhan, A, Stossi, F, Cecilia Ljungberg, M, Shelly, KE, Pew, BK, Solis, M, Rossano, AM, McAllister, JM, Wu, S & Richards, JAS 2019, 'VCAM1 is induced in ovarian theca and stromal cells in a mouse model of androgen excess', Endocrinology, vol. 160, no. 6, pp. 1377-1393. https://doi.org/10.1210/en.2018-00731
Candelaria NR, Padmanabhan A, Stossi F, Cecilia Ljungberg M, Shelly KE, Pew BK et al. VCAM1 is induced in ovarian theca and stromal cells in a mouse model of androgen excess. Endocrinology. 2019 Jun 1;160(6):1377-1393. https://doi.org/10.1210/en.2018-00731
Candelaria, Nicholes R. ; Padmanabhan, Achuth ; Stossi, Fabio ; Cecilia Ljungberg, M. ; Shelly, Katharine E. ; Pew, Braden K. ; Solis, Minerva ; Rossano, Ayane M. ; McAllister, Jan M. ; Wu, Sheng ; Richards, Jo Anne S. / VCAM1 is induced in ovarian theca and stromal cells in a mouse model of androgen excess. In: Endocrinology. 2019 ; Vol. 160, No. 6. pp. 1377-1393.
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AU - Padmanabhan, Achuth

AU - Stossi, Fabio

AU - Cecilia Ljungberg, M.

AU - Shelly, Katharine E.

AU - Pew, Braden K.

AU - Solis, Minerva

AU - Rossano, Ayane M.

AU - McAllister, Jan M.

AU - Wu, Sheng

AU - Richards, Jo Anne S.

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AB - Ovarian theca androgen production is regulated by the pituitary LH and intrafollicular factors. Enhanced androgen biosynthesis by theca cells contributes to polycystic ovary syndrome (PCOS) in women, but the ovarian consequences of elevated androgens are not completely understood. Our study documents the molecular events that are altered in the theca and stromal cells of mice exposed to high androgen levels, using the nonaromatizable androgen DHT. Changes in ovarian morphology and function were observed not only in follicles, but also in the stromal compartment. Genome-wide microarray analyses revealed marked changes in the ovarian transcriptome of DHT-treated females within 1 week. Particularly striking was the increased expression of vascular cell adhesion molecule 1 (Vcam1) specifically in the NR2F2/COUPTF-II lineage theca cells, not granulosa cells, of growing follicles and throughout the stroma of the androgen-treated mice. This response was mediated by androgen receptors (ARs) present in theca and stromal cells. Human theca-derived cultures expressed both ARs and NR2F2 that were nuclear. VCAM1 mRNA and protein were higher in PCOS-derived theca cells compared with control theca and reduced markedly by the AR antagonist flutamide. In the DHT-treated mice, VCAM1 was transiently induced by equine chorionic gonadotropin, when androgen and estrogen biosynthesis peak in preovulatory follicles, and was potently suppressed by a superovulatory dose of human chorionic gonadotropin. High levels of VCAM1 in the theca and interstitial cells of DHT-treated mice and in adult Leydig cells indicate that there may be novel functions for VCAM1 in reproductive tissues, including the gonads.

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