Vasoproliferation in the neonatal dog model of oxygen-induced retinopathy

D. Scott McLeod, Sylvia N. Crone, Gerard A. Lutty

Research output: Contribution to journalArticlepeer-review


Purpose. To examine the time course and relative degree of proliferative response associated with revascularization after hyperoxic insult in the dog model of oxygen-induced retinopathy. Methods. Mitotic cell profiles (MCPs) were counted in serial cross-sections of ADPase flatembedded retinas of air- reared control 8-, 15-, and 22-day-old dogs and of age-matched oxygen- treated animals (4 days, 100% oxygen) after return to normoxia. Sectioning and analysis were performed along the radial axis of the forming primary vasculature from optic nerve head to periphery. Results. In air-reared control animals, lumenal-associated cell mitosis was low, with an average of 9.6 MCPs/mm2 in the 22-day-old dogs. In oxygen-treated animals, however the number of lumenal-associated MCPs was significantly higher, with an average of 52.5 MCPs/mm2 of tissue in the 8-day-old dogs, 45.1 MCPs/mm2 in the 15- day-old dogs, and 26.8 MCPs/mm2 in the 22-day-old dogs. Additionally, extracellular spaces in avascular retina were obliterated in oxygen-treated animals. Conclusions. This study demonstrates that in the neonatal dog, revascularization after hyperoxic insult involves a period of marked vasoproliferation that peaks somewhere between 3 to 10 days after return to room air. Oxygen-induced changes in the extravascular milieu are likely to affect the patter of reforming vasculature and may restrict growth anteriorly.

Original languageEnglish (US)
Pages (from-to)1322-1333
Number of pages12
JournalInvestigative Ophthalmology and Visual Science
Issue number7
StatePublished - Jun 1996


  • Muller cells
  • astrocytes
  • development
  • neovascularization
  • retinopathy of prematurity

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience


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