Vascular smooth muscle NO exposure from intraerythrocytic SNOHb

A mathematical model

Kejing Chen, Roland N. Pittman, Aleksander S Popel

Research output: Contribution to journalArticle

Abstract

We previously constructed computational models based on the biochemical pathway analysis of different nitric oxide (NO) synthase isoforms and found a large discrepancy between our predictions and perivascular NO measurements, suggesting the existence of nonenzymatic sources of NO. S-nitrosohemoglobin (SNOHb) has been suggested as a major source to release NO in the arteriolar lumen and induce hypoxic vasodilation. In the present study, we formulated a multicellular computational model to quantify NO exposure in arteriolar smooth muscle when the NO released by intraerythrocytic SNOHb is the sole NO source in the vasculature. Our calculations show an NO exposure of ∼0.25-6 pM in the smooth muscle region. This amount does not account for the large discrepancy we encountered regarding perivascular NO levels. We also found that the amount of NO delivered by SNOHb to smooth muscle strongly depends on the SNOHb concentration and half-life, which further determine the rate of NO release, as well as on the membrane permeability of red blood cells (RBCs) to NO. In conclusion, our mathematical model predicts that picomolar amounts of NO can be delivered to the vascular smooth muscle by intraerythrocytic SNOHb; this amount of NO alone appears not sufficient to induce the hypoxic vasodilation.

Original languageEnglish (US)
Pages (from-to)1097-1110
Number of pages14
JournalAntioxidants and Redox Signaling
Volume9
Issue number8
DOIs
StatePublished - Aug 2007

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Vascular Smooth Muscle
Muscle
Nitric Oxide
Theoretical Models
Mathematical models
Smooth Muscle
Vasodilation
S-nitrosohemoglobin
Nitric Oxide Synthase
Half-Life
Permeability
Protein Isoforms
Blood
Erythrocytes
Cells
Membranes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Vascular smooth muscle NO exposure from intraerythrocytic SNOHb : A mathematical model. / Chen, Kejing; Pittman, Roland N.; Popel, Aleksander S.

In: Antioxidants and Redox Signaling, Vol. 9, No. 8, 08.2007, p. 1097-1110.

Research output: Contribution to journalArticle

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