Vascular Smooth Muscle Actions and Receptor Interactions of 8-Iso-prostaglandin E2, an E2-Isoprostane

M. Fukunaga, K. Takahashi, K. F. Badr

Research output: Contribution to journalArticle

Abstract

8-iso-PGE2, an E2-isoprostane, decreased GFR and RPF dose-dependently in rats, but with lesser potency than 8-epi-PGF, an F2-isoprostane. This effect was abolished by SQ29,548. 8-iso-PGE2 displaced [3H]SQ29,548 or [125I]BOP binding in aortic smooth muscle cells with the affinity rank order of SQ29,548 > = I-BOP > U46,619 > 8-iso-PGE2 > PGF, while it activated phospholipase C with a potency greater than those of I-BOP or U46,619 and lesser than that of 8-epi-PGF. We concluded that 8-iso-PGE2 is a renal vasoconstrictor linked to phosphoinositide metabolism. Its vascular smooth muscle contractile actions are likely mediated through activation of putative thromboxane A2 receptor-like "isoprostane receptors".

Original languageEnglish (US)
Pages (from-to)507-515
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume195
Issue number2
DOIs
StatePublished - Sep 15 1993
Externally publishedYes

Fingerprint

Isoprostanes
Vascular Smooth Muscle
Dinoprostone
Dinoprost
Muscle
F2-Isoprostanes
Prostaglandin H2 Receptors Thromboxane A2
Type C Phospholipases
Vasoconstrictor Agents
Phosphatidylinositols
Metabolism
Smooth Muscle Myocytes
Rats
Chemical activation
Cells
Kidney
8-isoprostaglandin E2
7-(3-(3-hydroxy-4-(4'-iodophenoxy)-1-butenyl)-7-oxabicyclo(2.2.1)heptan-2-yl)-5-heptenoic acid

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Cite this

Vascular Smooth Muscle Actions and Receptor Interactions of 8-Iso-prostaglandin E2, an E2-Isoprostane. / Fukunaga, M.; Takahashi, K.; Badr, K. F.

In: Biochemical and Biophysical Research Communications, Vol. 195, No. 2, 15.09.1993, p. 507-515.

Research output: Contribution to journalArticle

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