Vascular progenitors from cord blood-derived induced pluripotent stem cells possess augmented capacity for regenerating ischemic retinal vasculature

Tea Soon Park, Imran Bhutto, Ludovic Zimmerlin, Jeffrey Huo, Pratik Nagaria, Diana Miller, Abdul Jalil Rufaihah, Connie Talbot, Jack Aguilar, Rhonda Grebe, Carol Merges, Renee Reijo-Pera, Ricardo A. Feldman, Feyruz Rassool, John Cooke, Gerard Anthony Lutty, Elias Zambidis

Research output: Contribution to journalArticle

Abstract

Background-The generation of vascular progenitors (VPs) from human induced pluripotent stem cells (hiPSCs) has great potential for treating vascular disorders such as ischemic retinopathies. However, long-term in vivo engraftment of hiPSCderived VPs into the retina has not yet been reported. This goal may be limited by the low differentiation yield, greater senescence, and poor proliferation of hiPSC-derived vascular cells. To evaluate the potential of hiPSCs for treating ischemic retinopathies, we generated VPs from a repertoire of viral-integrated and nonintegrated fibroblast and cord blood (CB)-derived hiPSC lines and tested their capacity for homing and engrafting into murine retina in an ischemia-reperfusion model. Methods and Results-VPs from human embryonic stem cells and hiPSCs were generated with an optimized vascular differentiation system. Fluorescence-activated cell sorting purification of human embryoid body cells differentially expressing endothelial/pericytic markers identified a CD31+CD146+ VP population with high vascular potency. Episomal CB-induced pluripotent stem cells (iPSCs) generated these VPs with higher efficiencies than fibroblast-iPSC. Moreover, in contrast to fibroblast-iPSC-VPs, CB-iPSC-VPs maintained expression signatures more comparable to human embryonic stem cell VPs, expressed higher levels of immature vascular markers, demonstrated less culture senescence and sensitivity to DNA damage, and possessed fewer transmitted reprogramming errors. Luciferase transgene-marked VPs from human embryonic stem cells, CB-iPSCs, and fibroblast-iPSCs were injected systemically or directly into the vitreous of retinal ischemia-reperfusion-injured adult nonobese diabetic-severe combined immunodeficient mice. Only human embryonic stem cell- and CB-iPSC-derived VPs reliably homed and engrafted into injured retinal capillaries, with incorporation into damaged vessels for up to 45 days. Conclusions-VPs generated from CB-iPSCs possessed augmented capacity to home, integrate into, and repair damaged retinal vasculature.

Original languageEnglish (US)
Pages (from-to)359-372
Number of pages14
JournalCirculation
Volume129
Issue number3
DOIs
StatePublished - Jan 21 2014

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Induced Pluripotent Stem Cells
Fetal Blood
Blood Vessels
Fibroblasts
Reperfusion
Retina
Ischemia
Embryoid Bodies
SCID Mice

Keywords

  • Blood supply
  • Diabetic retinopathy
  • Embryonic stem cells
  • Induced pluripotent stem cells
  • Reperfusion injury
  • Stem cells
  • Transplantation

ASJC Scopus subject areas

  • Physiology (medical)
  • Cardiology and Cardiovascular Medicine

Cite this

Vascular progenitors from cord blood-derived induced pluripotent stem cells possess augmented capacity for regenerating ischemic retinal vasculature. / Park, Tea Soon; Bhutto, Imran; Zimmerlin, Ludovic; Huo, Jeffrey; Nagaria, Pratik; Miller, Diana; Rufaihah, Abdul Jalil; Talbot, Connie; Aguilar, Jack; Grebe, Rhonda; Merges, Carol; Reijo-Pera, Renee; Feldman, Ricardo A.; Rassool, Feyruz; Cooke, John; Lutty, Gerard Anthony; Zambidis, Elias.

In: Circulation, Vol. 129, No. 3, 21.01.2014, p. 359-372.

Research output: Contribution to journalArticle

Park, TS, Bhutto, I, Zimmerlin, L, Huo, J, Nagaria, P, Miller, D, Rufaihah, AJ, Talbot, C, Aguilar, J, Grebe, R, Merges, C, Reijo-Pera, R, Feldman, RA, Rassool, F, Cooke, J, Lutty, GA & Zambidis, E 2014, 'Vascular progenitors from cord blood-derived induced pluripotent stem cells possess augmented capacity for regenerating ischemic retinal vasculature', Circulation, vol. 129, no. 3, pp. 359-372. https://doi.org/10.1161/CIRCULATIONAHA.113.003000
Park, Tea Soon ; Bhutto, Imran ; Zimmerlin, Ludovic ; Huo, Jeffrey ; Nagaria, Pratik ; Miller, Diana ; Rufaihah, Abdul Jalil ; Talbot, Connie ; Aguilar, Jack ; Grebe, Rhonda ; Merges, Carol ; Reijo-Pera, Renee ; Feldman, Ricardo A. ; Rassool, Feyruz ; Cooke, John ; Lutty, Gerard Anthony ; Zambidis, Elias. / Vascular progenitors from cord blood-derived induced pluripotent stem cells possess augmented capacity for regenerating ischemic retinal vasculature. In: Circulation. 2014 ; Vol. 129, No. 3. pp. 359-372.
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AU - Park, Tea Soon

AU - Bhutto, Imran

AU - Zimmerlin, Ludovic

AU - Huo, Jeffrey

AU - Nagaria, Pratik

AU - Miller, Diana

AU - Rufaihah, Abdul Jalil

AU - Talbot, Connie

AU - Aguilar, Jack

AU - Grebe, Rhonda

AU - Merges, Carol

AU - Reijo-Pera, Renee

AU - Feldman, Ricardo A.

AU - Rassool, Feyruz

AU - Cooke, John

AU - Lutty, Gerard Anthony

AU - Zambidis, Elias

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N2 - Background-The generation of vascular progenitors (VPs) from human induced pluripotent stem cells (hiPSCs) has great potential for treating vascular disorders such as ischemic retinopathies. However, long-term in vivo engraftment of hiPSCderived VPs into the retina has not yet been reported. This goal may be limited by the low differentiation yield, greater senescence, and poor proliferation of hiPSC-derived vascular cells. To evaluate the potential of hiPSCs for treating ischemic retinopathies, we generated VPs from a repertoire of viral-integrated and nonintegrated fibroblast and cord blood (CB)-derived hiPSC lines and tested their capacity for homing and engrafting into murine retina in an ischemia-reperfusion model. Methods and Results-VPs from human embryonic stem cells and hiPSCs were generated with an optimized vascular differentiation system. Fluorescence-activated cell sorting purification of human embryoid body cells differentially expressing endothelial/pericytic markers identified a CD31+CD146+ VP population with high vascular potency. Episomal CB-induced pluripotent stem cells (iPSCs) generated these VPs with higher efficiencies than fibroblast-iPSC. Moreover, in contrast to fibroblast-iPSC-VPs, CB-iPSC-VPs maintained expression signatures more comparable to human embryonic stem cell VPs, expressed higher levels of immature vascular markers, demonstrated less culture senescence and sensitivity to DNA damage, and possessed fewer transmitted reprogramming errors. Luciferase transgene-marked VPs from human embryonic stem cells, CB-iPSCs, and fibroblast-iPSCs were injected systemically or directly into the vitreous of retinal ischemia-reperfusion-injured adult nonobese diabetic-severe combined immunodeficient mice. Only human embryonic stem cell- and CB-iPSC-derived VPs reliably homed and engrafted into injured retinal capillaries, with incorporation into damaged vessels for up to 45 days. Conclusions-VPs generated from CB-iPSCs possessed augmented capacity to home, integrate into, and repair damaged retinal vasculature.

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