Vascular perfused rat phrenic nerve-hemidiaphragm. A model system for studying the physiological and neurochemical aspects of neuromuscular transmission

G. G. Bierkamper, Alan Goldberg

Research output: Contribution to journalArticle

Abstract

A model system has been developed for studying the release of endogenous acetylcholine from the vascular perfused phrenic nerve-hemidiaphragm of the rat. A method is described for cannulating the diaphragmatic vein. This cannulated preparation is placed in a specially designed Plexiglass chamber. The small volume of perfusion necessary to maintain the preparation facilitates the microanalysis of released endogenous or exogenous compounds. Further, the perfusion system increases the viability and stability of the preparation for at least 6 hours. Using a slightly modified chamber, intracelluar recordings can be made coincident with chemical measurements.

Original languageEnglish (US)
Pages (from-to)40-46
Number of pages7
JournalJournal of Electrophysiological Techniques
Volume6
Issue number2
StatePublished - 1978

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Phrenic Nerve
Blood Vessels
Perfusion
Polymethyl Methacrylate
Acetylcholine
Veins

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

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abstract = "A model system has been developed for studying the release of endogenous acetylcholine from the vascular perfused phrenic nerve-hemidiaphragm of the rat. A method is described for cannulating the diaphragmatic vein. This cannulated preparation is placed in a specially designed Plexiglass chamber. The small volume of perfusion necessary to maintain the preparation facilitates the microanalysis of released endogenous or exogenous compounds. Further, the perfusion system increases the viability and stability of the preparation for at least 6 hours. Using a slightly modified chamber, intracelluar recordings can be made coincident with chemical measurements.",
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