Vascular gene expression in nonneoplastic and malignant brain

Stephen L. Madden, Brian P. Cook, Mariana Nacht, William D. Weber, Michelle R. Callahan, Yide Jiang, Michael R. Dufault, Xiaoming Zhang, Wen Zhang, Jennifer Walter-Yohrling, Cecile Rouleau, Viatcheslav R. Akmaev, Clarence J. Wang, Xiaohong Cao, Thia B. St. Martin, Bruce L. Roberts, Beverly A. Teicher, Katherine W. Klinger, Radu Virgil Stan, Brenden LuceyEleanor B. Carson-Walter, John J Laterra, Kevin A. Walter

Research output: Contribution to journalArticle

Abstract

Malignant gliomas are uniformly lethal tumors whose morbidity is mediated in large part by the angiogenic response of the brain to the invading tumor. This profound angiogenic response leads to aggressive tumor invasion and destruction of surrounding brain tissue as well as blood-brain barrier breakdown and life-threatening cerebral edema. To investigate the molecular mechanisms governing the proliferation of abnormal microvasculature in malignant brain tumor patients, we have undertaken a cell-specific transcriptome analysis from surgically harvested nonneoplastic and tumor-associated endothelial cells. SAGE-derived endothelial cell gene expression patterns from glioma and nonneoplastic brain tissue reveal distinct gene expression patterns and consistent up-regulation of certain glioma endothelial marker genes across patient samples. We define the G-protein-coupled receptor RDC1 as a tumor endothelial marker whose expression is distinctly induced in tumor endothelial cells of both brain and peripheral vasculature. Further, we demonstrate that the glioma-induced gene, PV1, shows expression both restricted to endothelial cells and coincident with endothelial cell tube formation. As PV1 provides a framework for endothelial cell caveolar diaphragms, this protein may serve to enhance glioma-induced disruption of the blood-brain barrier and transendothelial exchange. Additional characterization of this extensive brain endothelial cell gene expression database will provide unique molecular insights into vascular gene expression.

Original languageEnglish (US)
Pages (from-to)601-608
Number of pages8
JournalAmerican Journal of Pathology
Volume165
Issue number2
StatePublished - Aug 2004

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Blood Vessels
Endothelial Cells
Glioma
Gene Expression
Brain
Neoplasms
Blood-Brain Barrier
Brain Edema
Gene Expression Profiling
Tumor Biomarkers
G-Protein-Coupled Receptors
Microvessels
Diaphragm
Brain Neoplasms
Genes
Up-Regulation
Databases
Morbidity
Proteins

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Madden, S. L., Cook, B. P., Nacht, M., Weber, W. D., Callahan, M. R., Jiang, Y., ... Walter, K. A. (2004). Vascular gene expression in nonneoplastic and malignant brain. American Journal of Pathology, 165(2), 601-608.

Vascular gene expression in nonneoplastic and malignant brain. / Madden, Stephen L.; Cook, Brian P.; Nacht, Mariana; Weber, William D.; Callahan, Michelle R.; Jiang, Yide; Dufault, Michael R.; Zhang, Xiaoming; Zhang, Wen; Walter-Yohrling, Jennifer; Rouleau, Cecile; Akmaev, Viatcheslav R.; Wang, Clarence J.; Cao, Xiaohong; St. Martin, Thia B.; Roberts, Bruce L.; Teicher, Beverly A.; Klinger, Katherine W.; Stan, Radu Virgil; Lucey, Brenden; Carson-Walter, Eleanor B.; Laterra, John J; Walter, Kevin A.

In: American Journal of Pathology, Vol. 165, No. 2, 08.2004, p. 601-608.

Research output: Contribution to journalArticle

Madden, SL, Cook, BP, Nacht, M, Weber, WD, Callahan, MR, Jiang, Y, Dufault, MR, Zhang, X, Zhang, W, Walter-Yohrling, J, Rouleau, C, Akmaev, VR, Wang, CJ, Cao, X, St. Martin, TB, Roberts, BL, Teicher, BA, Klinger, KW, Stan, RV, Lucey, B, Carson-Walter, EB, Laterra, JJ & Walter, KA 2004, 'Vascular gene expression in nonneoplastic and malignant brain', American Journal of Pathology, vol. 165, no. 2, pp. 601-608.
Madden SL, Cook BP, Nacht M, Weber WD, Callahan MR, Jiang Y et al. Vascular gene expression in nonneoplastic and malignant brain. American Journal of Pathology. 2004 Aug;165(2):601-608.
Madden, Stephen L. ; Cook, Brian P. ; Nacht, Mariana ; Weber, William D. ; Callahan, Michelle R. ; Jiang, Yide ; Dufault, Michael R. ; Zhang, Xiaoming ; Zhang, Wen ; Walter-Yohrling, Jennifer ; Rouleau, Cecile ; Akmaev, Viatcheslav R. ; Wang, Clarence J. ; Cao, Xiaohong ; St. Martin, Thia B. ; Roberts, Bruce L. ; Teicher, Beverly A. ; Klinger, Katherine W. ; Stan, Radu Virgil ; Lucey, Brenden ; Carson-Walter, Eleanor B. ; Laterra, John J ; Walter, Kevin A. / Vascular gene expression in nonneoplastic and malignant brain. In: American Journal of Pathology. 2004 ; Vol. 165, No. 2. pp. 601-608.
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