Abstract
Purpose. Vascular endothelial growth factor/vascular permeability factor (VEGF/VPF) is an important mediator of the intraocular neovascularization common to numerous ischemic retinopathies. Increased vascular permeability frequently accompanies these conditions. Although VEGF is a potent vasopermeability factor in skin, its permeability effects on the retinal vasculature are not well understood. We evaluated retinal vascular permeability in rats following intravitreal and intravenous administration of VEGF. Methods. Sprague-Dawley rats had jugular catheters placed 24 hours prior to each experiment. For intravitreal studies, each rat received a 10ul intravitreal injection of VEGF in one eye and a similar injection of PBS in the contralateral eye. Retinal vascular integrity was evaluated by SLO following the injections. A 30ul bolus of fluorescein was delivered through the catheter after various periods and aqueous and vitreous fluorophotometry were performed 0, 2, 5, 10, 15, 2) and 30 minutes later. For intravenous studies, a VRGF bolus was injected through the catheter and subsequent fluorophotometry performed. Results. Intravitreal injection of VEGF increased retinal vascular permeability in a dose dependent manner attaining levels 2.7 fold higher than control eyes within 5 minutes (final intraocular VEGF = 50ng/ml). The effect was evident at all time points (P
| Original language | English (US) |
|---|---|
| Journal | Investigative Ophthalmology and Visual Science |
| Volume | 37 |
| Issue number | 3 |
| State | Published - Feb 15 1996 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Ophthalmology
Cite this
Vascular Endothelial Growth Factor (VEGF) increases retinal vascular permeability in vivo. / Duh, Elia J; Aiello, L. P.; Takagi, C.; Clermont, A.; Mori, F.; King, G. L.; Bursel, S. E.
In: Investigative Ophthalmology and Visual Science, Vol. 37, No. 3, 15.02.1996.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Vascular Endothelial Growth Factor (VEGF) increases retinal vascular permeability in vivo
AU - Duh, Elia J
AU - Aiello, L. P.
AU - Takagi, C.
AU - Clermont, A.
AU - Mori, F.
AU - King, G. L.
AU - Bursel, S. E.
PY - 1996/2/15
Y1 - 1996/2/15
N2 - Purpose. Vascular endothelial growth factor/vascular permeability factor (VEGF/VPF) is an important mediator of the intraocular neovascularization common to numerous ischemic retinopathies. Increased vascular permeability frequently accompanies these conditions. Although VEGF is a potent vasopermeability factor in skin, its permeability effects on the retinal vasculature are not well understood. We evaluated retinal vascular permeability in rats following intravitreal and intravenous administration of VEGF. Methods. Sprague-Dawley rats had jugular catheters placed 24 hours prior to each experiment. For intravitreal studies, each rat received a 10ul intravitreal injection of VEGF in one eye and a similar injection of PBS in the contralateral eye. Retinal vascular integrity was evaluated by SLO following the injections. A 30ul bolus of fluorescein was delivered through the catheter after various periods and aqueous and vitreous fluorophotometry were performed 0, 2, 5, 10, 15, 2) and 30 minutes later. For intravenous studies, a VRGF bolus was injected through the catheter and subsequent fluorophotometry performed. Results. Intravitreal injection of VEGF increased retinal vascular permeability in a dose dependent manner attaining levels 2.7 fold higher than control eyes within 5 minutes (final intraocular VEGF = 50ng/ml). The effect was evident at all time points (P
AB - Purpose. Vascular endothelial growth factor/vascular permeability factor (VEGF/VPF) is an important mediator of the intraocular neovascularization common to numerous ischemic retinopathies. Increased vascular permeability frequently accompanies these conditions. Although VEGF is a potent vasopermeability factor in skin, its permeability effects on the retinal vasculature are not well understood. We evaluated retinal vascular permeability in rats following intravitreal and intravenous administration of VEGF. Methods. Sprague-Dawley rats had jugular catheters placed 24 hours prior to each experiment. For intravitreal studies, each rat received a 10ul intravitreal injection of VEGF in one eye and a similar injection of PBS in the contralateral eye. Retinal vascular integrity was evaluated by SLO following the injections. A 30ul bolus of fluorescein was delivered through the catheter after various periods and aqueous and vitreous fluorophotometry were performed 0, 2, 5, 10, 15, 2) and 30 minutes later. For intravenous studies, a VRGF bolus was injected through the catheter and subsequent fluorophotometry performed. Results. Intravitreal injection of VEGF increased retinal vascular permeability in a dose dependent manner attaining levels 2.7 fold higher than control eyes within 5 minutes (final intraocular VEGF = 50ng/ml). The effect was evident at all time points (P
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M3 - Article
AN - SCOPUS:1842565896
VL - 37
JO - Investigative Ophthalmology and Visual Science
JF - Investigative Ophthalmology and Visual Science
SN - 0146-0404
IS - 3
ER -