Vascular Endothelial Growth Factor (VEGF) increases retinal vascular permeability in vivo

Elia J Duh, L. P. Aiello, C. Takagi, A. Clermont, F. Mori, G. L. King, S. E. Bursel

Research output: Contribution to journalArticle

Abstract

Purpose. Vascular endothelial growth factor/vascular permeability factor (VEGF/VPF) is an important mediator of the intraocular neovascularization common to numerous ischemic retinopathies. Increased vascular permeability frequently accompanies these conditions. Although VEGF is a potent vasopermeability factor in skin, its permeability effects on the retinal vasculature are not well understood. We evaluated retinal vascular permeability in rats following intravitreal and intravenous administration of VEGF. Methods. Sprague-Dawley rats had jugular catheters placed 24 hours prior to each experiment. For intravitreal studies, each rat received a 10ul intravitreal injection of VEGF in one eye and a similar injection of PBS in the contralateral eye. Retinal vascular integrity was evaluated by SLO following the injections. A 30ul bolus of fluorescein was delivered through the catheter after various periods and aqueous and vitreous fluorophotometry were performed 0, 2, 5, 10, 15, 2) and 30 minutes later. For intravenous studies, a VRGF bolus was injected through the catheter and subsequent fluorophotometry performed. Results. Intravitreal injection of VEGF increased retinal vascular permeability in a dose dependent manner attaining levels 2.7 fold higher than control eyes within 5 minutes (final intraocular VEGF = 50ng/ml). The effect was evident at all time points (P

Original languageEnglish (US)
JournalInvestigative Ophthalmology and Visual Science
Volume37
Issue number3
StatePublished - Feb 15 1996
Externally publishedYes

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Retinal Vessels
Capillary Permeability
Vascular Endothelial Growth Factor A
Fluorophotometry
Intravitreal Injections
Catheters
Injections
Fluorescein
Intravenous Administration
Sprague Dawley Rats
Permeability
Neck
Skin

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Duh, E. J., Aiello, L. P., Takagi, C., Clermont, A., Mori, F., King, G. L., & Bursel, S. E. (1996). Vascular Endothelial Growth Factor (VEGF) increases retinal vascular permeability in vivo. Investigative Ophthalmology and Visual Science, 37(3).

Vascular Endothelial Growth Factor (VEGF) increases retinal vascular permeability in vivo. / Duh, Elia J; Aiello, L. P.; Takagi, C.; Clermont, A.; Mori, F.; King, G. L.; Bursel, S. E.

In: Investigative Ophthalmology and Visual Science, Vol. 37, No. 3, 15.02.1996.

Research output: Contribution to journalArticle

Duh, EJ, Aiello, LP, Takagi, C, Clermont, A, Mori, F, King, GL & Bursel, SE 1996, 'Vascular Endothelial Growth Factor (VEGF) increases retinal vascular permeability in vivo', Investigative Ophthalmology and Visual Science, vol. 37, no. 3.
Duh, Elia J ; Aiello, L. P. ; Takagi, C. ; Clermont, A. ; Mori, F. ; King, G. L. ; Bursel, S. E. / Vascular Endothelial Growth Factor (VEGF) increases retinal vascular permeability in vivo. In: Investigative Ophthalmology and Visual Science. 1996 ; Vol. 37, No. 3.
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