Vascular effects following homozygous disruption of p47(phox)

An essential component of NADPH oxidase

Eileen Hsich, Brahm H. Segal, Patrick J. Pagano, Federico E. Rey, Beverly Paigen, John Deleonardis, Robert F. Hoyt, Steven M. Holland, Toren Finkel

Research output: Contribution to journalArticle

Abstract

Background - Evidence suggests that the vessel wall contains an oxidase similar, if not identical, to phagocytic NADPH oxidase. We tested the contribution of this specific oxidase to the progression of atherosclerosis and the regulation of blood pressure. Methods and Results - An examination of aortic rings from wild-type mice and mice with homozygous targeted disruptions in p47(phox) revealed that p47(phox) knockout mice had a reduction in vascular superoxide production. However, analyses of apoE -/- p47(phox)+/+ and apoE -/- p47(phox) -/- strains of mice demonstrated no significant differences in atherosclerotic lesion sizes. Similarly, analyses of wild-type and p47(phox) knockout mice revealed no differences in either basal blood pressure or the rise in blood pressure seen after the pharmacological inhibition of nitric oxide synthase. Conclusions - NADPH oxidase contributes to basal vascular superoxide production. However, the absence of a functional oxidase does not significantly affect the progression of atherosclerosis in the standard mouse apoE -/- model, nor does it significantly influence basal blood pressure.

Original languageEnglish (US)
Pages (from-to)1234-1236
Number of pages3
JournalCirculation
Volume101
Issue number11
StatePublished - Mar 21 2000
Externally publishedYes

Fingerprint

NADPH Oxidase
Blood Vessels
Apolipoproteins E
Blood Pressure
Oxidoreductases
Knockout Mice
Superoxides
Atherosclerosis
Nitric Oxide Synthase
4-ethoxymethylene-2-phenyl-2-oxazoline-5-one
Pharmacology

Keywords

  • Apolipoproteins
  • Atherosclerosis
  • Blood pressure

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Hsich, E., Segal, B. H., Pagano, P. J., Rey, F. E., Paigen, B., Deleonardis, J., ... Finkel, T. (2000). Vascular effects following homozygous disruption of p47(phox): An essential component of NADPH oxidase. Circulation, 101(11), 1234-1236.

Vascular effects following homozygous disruption of p47(phox) : An essential component of NADPH oxidase. / Hsich, Eileen; Segal, Brahm H.; Pagano, Patrick J.; Rey, Federico E.; Paigen, Beverly; Deleonardis, John; Hoyt, Robert F.; Holland, Steven M.; Finkel, Toren.

In: Circulation, Vol. 101, No. 11, 21.03.2000, p. 1234-1236.

Research output: Contribution to journalArticle

Hsich, E, Segal, BH, Pagano, PJ, Rey, FE, Paigen, B, Deleonardis, J, Hoyt, RF, Holland, SM & Finkel, T 2000, 'Vascular effects following homozygous disruption of p47(phox): An essential component of NADPH oxidase', Circulation, vol. 101, no. 11, pp. 1234-1236.
Hsich E, Segal BH, Pagano PJ, Rey FE, Paigen B, Deleonardis J et al. Vascular effects following homozygous disruption of p47(phox): An essential component of NADPH oxidase. Circulation. 2000 Mar 21;101(11):1234-1236.
Hsich, Eileen ; Segal, Brahm H. ; Pagano, Patrick J. ; Rey, Federico E. ; Paigen, Beverly ; Deleonardis, John ; Hoyt, Robert F. ; Holland, Steven M. ; Finkel, Toren. / Vascular effects following homozygous disruption of p47(phox) : An essential component of NADPH oxidase. In: Circulation. 2000 ; Vol. 101, No. 11. pp. 1234-1236.
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AB - Background - Evidence suggests that the vessel wall contains an oxidase similar, if not identical, to phagocytic NADPH oxidase. We tested the contribution of this specific oxidase to the progression of atherosclerosis and the regulation of blood pressure. Methods and Results - An examination of aortic rings from wild-type mice and mice with homozygous targeted disruptions in p47(phox) revealed that p47(phox) knockout mice had a reduction in vascular superoxide production. However, analyses of apoE -/- p47(phox)+/+ and apoE -/- p47(phox) -/- strains of mice demonstrated no significant differences in atherosclerotic lesion sizes. Similarly, analyses of wild-type and p47(phox) knockout mice revealed no differences in either basal blood pressure or the rise in blood pressure seen after the pharmacological inhibition of nitric oxide synthase. Conclusions - NADPH oxidase contributes to basal vascular superoxide production. However, the absence of a functional oxidase does not significantly affect the progression of atherosclerosis in the standard mouse apoE -/- model, nor does it significantly influence basal blood pressure.

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