Varying Degrees of Amplification of the N-ras Oncogene in the Human Breast Cancer Cell Line MCF-7

Kathryn A. Graham, Carol L. Richardson, Mark D. Minden, Ronald N. Buick, Jeffrey M. Trent

Research output: Contribution to journalArticlepeer-review

Abstract

The oncogene N-ras has been found to be amplified (20 copies) in the human breast carcinoma cell line MCF-7. The amplified sequences have been localized to a marker chromosome by in situ hybridization. Sublines of MCF-7, serially passaged in different laboratories; have marked variation in the degree of N-ras amplification. The differing degrees of amplification of N-ras are further evidence of heterogeneity within MCF-7 subclones. The phenomenon may not have general relevance for breast cancer, since other breast cancer cell lines and DNA from patient biopsies failed to show evidence of N-ras amplification.

Original languageEnglish (US)
Pages (from-to)2201-2205
Number of pages5
JournalCancer Research
Volume45
Issue number5
StatePublished - May 1 1985

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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