Variations in stromal signatures in breast and colorectal cancer metastases

Jonathan Webster, Andrew H. Beck, Mimansa Sharma, Inigo Espinosa, Britta Weigelt, Marthe Schreuder, Kelli D. Montgomery, Kristin C. Jensen, Matt Van De Rijn, Robert West

Research output: Contribution to journalArticle

Abstract

The tumour microenvironment (TME) plays an important role in tumour survival and growth, but little is known about the degree of preservation between different stromal response patterns found in primary tumours and their metastases. We have previously identified gene expression profiles for two distinct stromal signatures in breast carcinoma of fibroblast (aka DTF) and macrophage (aka CSF1) response and found them to be correlated with clinicopathological features, including outcome. In this study, we compare the DTF fibroblast and CSF1 macrophage stromal response patterns in primary breast and colorectal cancers to their matched lymph node metastases. In both breast and colorectal cancer, there was a significant positive correlation between the CSF1 macrophage signature in the primary tumours and the matched lymph node metastases, as assessed by immunohistochemical markers. No such correlation was observed for the DTF fibroblast signature. A similar result was seen in independent analysis of two published gene expression microarray datasets. The variations of these stromal reaction patterns from the primary to the metastasis shed light on the relationship between the neoplastic cells and the non-neoplastic cells in the TME. The preservation of the CSF1 macrophage response pattern in metastases lends support to targeting the CSF1 pathway in cancer.

Original languageEnglish (US)
Pages (from-to)158-165
Number of pages8
JournalJournal of Pathology
Volume222
Issue number2
DOIs
Publication statusPublished - Oct 1 2010
Externally publishedYes

    Fingerprint

Keywords

  • Breast cancer
  • Gene expression
  • Metastasis
  • Tumour microenvironment

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Webster, J., Beck, A. H., Sharma, M., Espinosa, I., Weigelt, B., Schreuder, M., ... West, R. (2010). Variations in stromal signatures in breast and colorectal cancer metastases. Journal of Pathology, 222(2), 158-165. https://doi.org/10.1002/path.2738