Variation in large-conductance, calcium-activated potassium channels from hair cells along the chicken basilar papilla

R. K. Duncan, Paul Albert Fuchs

Research output: Contribution to journalArticle

Abstract

The mechanism for electrical tuning in non-mammalian hair cells rests within the widely diverse kinetics of functionally distinct, large-conductance potassium channels (BK), thought to result from alternative splicing of the pore-forming α subunit and variable co-expression with an accessory β subunit. Inside-out patches from hair cells along the chicken basilar papilla revealed 'tonotopic' gradations in calcium sensitivity and deactivation kinetics. The resonant frequency for the hair cell from which the patch was taken was estimated from deactivation rates, and this frequency reasonably matched that predicted from the originating cell's tonotopic location. The rates of deactivation for native BK channels were much faster than rates reported for cloned chicken BK channels including both α and β subunits. This result was surprising since patches were pulled from hair cells in the apical half of the papilla where β subunits are most highly expressed. Heterogeneity in the properties of native chicken BK channels implies a high degree of molecular variation and hinders our ability to identify those molecular constituents.

Original languageEnglish (US)
Pages (from-to)357-371
Number of pages15
JournalJournal of Physiology
Volume547
Issue number2
DOIs
StatePublished - Mar 1 2003

Fingerprint

Large-Conductance Calcium-Activated Potassium Channels
Organ of Corti
Chickens
Potassium Channels
Alternative Splicing
Calcium

ASJC Scopus subject areas

  • Physiology

Cite this

Variation in large-conductance, calcium-activated potassium channels from hair cells along the chicken basilar papilla. / Duncan, R. K.; Fuchs, Paul Albert.

In: Journal of Physiology, Vol. 547, No. 2, 01.03.2003, p. 357-371.

Research output: Contribution to journalArticle

@article{76a5e48116da40718f1382d94b9d6ae7,
title = "Variation in large-conductance, calcium-activated potassium channels from hair cells along the chicken basilar papilla",
abstract = "The mechanism for electrical tuning in non-mammalian hair cells rests within the widely diverse kinetics of functionally distinct, large-conductance potassium channels (BK), thought to result from alternative splicing of the pore-forming α subunit and variable co-expression with an accessory β subunit. Inside-out patches from hair cells along the chicken basilar papilla revealed 'tonotopic' gradations in calcium sensitivity and deactivation kinetics. The resonant frequency for the hair cell from which the patch was taken was estimated from deactivation rates, and this frequency reasonably matched that predicted from the originating cell's tonotopic location. The rates of deactivation for native BK channels were much faster than rates reported for cloned chicken BK channels including both α and β subunits. This result was surprising since patches were pulled from hair cells in the apical half of the papilla where β subunits are most highly expressed. Heterogeneity in the properties of native chicken BK channels implies a high degree of molecular variation and hinders our ability to identify those molecular constituents.",
author = "Duncan, {R. K.} and Fuchs, {Paul Albert}",
year = "2003",
month = "3",
day = "1",
doi = "10.1113/jphysiol.2002.029785",
language = "English (US)",
volume = "547",
pages = "357--371",
journal = "Journal of Physiology",
issn = "0022-3751",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Variation in large-conductance, calcium-activated potassium channels from hair cells along the chicken basilar papilla

AU - Duncan, R. K.

AU - Fuchs, Paul Albert

PY - 2003/3/1

Y1 - 2003/3/1

N2 - The mechanism for electrical tuning in non-mammalian hair cells rests within the widely diverse kinetics of functionally distinct, large-conductance potassium channels (BK), thought to result from alternative splicing of the pore-forming α subunit and variable co-expression with an accessory β subunit. Inside-out patches from hair cells along the chicken basilar papilla revealed 'tonotopic' gradations in calcium sensitivity and deactivation kinetics. The resonant frequency for the hair cell from which the patch was taken was estimated from deactivation rates, and this frequency reasonably matched that predicted from the originating cell's tonotopic location. The rates of deactivation for native BK channels were much faster than rates reported for cloned chicken BK channels including both α and β subunits. This result was surprising since patches were pulled from hair cells in the apical half of the papilla where β subunits are most highly expressed. Heterogeneity in the properties of native chicken BK channels implies a high degree of molecular variation and hinders our ability to identify those molecular constituents.

AB - The mechanism for electrical tuning in non-mammalian hair cells rests within the widely diverse kinetics of functionally distinct, large-conductance potassium channels (BK), thought to result from alternative splicing of the pore-forming α subunit and variable co-expression with an accessory β subunit. Inside-out patches from hair cells along the chicken basilar papilla revealed 'tonotopic' gradations in calcium sensitivity and deactivation kinetics. The resonant frequency for the hair cell from which the patch was taken was estimated from deactivation rates, and this frequency reasonably matched that predicted from the originating cell's tonotopic location. The rates of deactivation for native BK channels were much faster than rates reported for cloned chicken BK channels including both α and β subunits. This result was surprising since patches were pulled from hair cells in the apical half of the papilla where β subunits are most highly expressed. Heterogeneity in the properties of native chicken BK channels implies a high degree of molecular variation and hinders our ability to identify those molecular constituents.

UR - http://www.scopus.com/inward/record.url?scp=0037338180&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037338180&partnerID=8YFLogxK

U2 - 10.1113/jphysiol.2002.029785

DO - 10.1113/jphysiol.2002.029785

M3 - Article

C2 - 12562934

AN - SCOPUS:0037338180

VL - 547

SP - 357

EP - 371

JO - Journal of Physiology

JF - Journal of Physiology

SN - 0022-3751

IS - 2

ER -