Variation in hemoglobin F production among normal and sickle cell adults is not related to nucleotide substitutions in the gamma promoter regions

E. P. Economou, S. E. Antonarakis, H. H. Kazazian, G. R. Sergeant, G. J. Dover

Research output: Contribution to journalArticlepeer-review

Abstract

Single nucleotide substitutions in the promoter regions of the (A)γ- and (G)γ-globin genes have been associated with increased fetal hemoglobin (HbF) production. We wished to determine whether these or other unrecognized substitutions in the γ promoter regions are responsible for the 20-fold variation in HbF production in sickle cell patients or normal adults. From a random sampling of 250 sickle cell (SS) patients and 125 normal adults, 17 individuals representing the highest and lowest HbF producers were selected for study. All three common restriction fragment length polymorphism β-globin region haplotypes (Benin, Central African Republic, and Senegal) were found in both the highest and lowest HbF producers with SS disease. Using the polymerase chain reaction amplification and direct sequencing of the amplified DNA product, we examined the promoter regions of both the (A)γ and (G)γ genes from -350 bp to +50 bp of the CAP site. No mutations were found in either γ gene promoter region. We conclude that nucleotide substitutions in the promoter regions (-350 to +50 bp) of both γ genes are not responsible for the marked variation in HbF production among SS or normal individuals.

Original languageEnglish (US)
Pages (from-to)174-177
Number of pages4
JournalBlood
Volume77
Issue number1
DOIs
StatePublished - 1991

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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