Variation at the capsule locus, cps, of mistyped and non-typable Streptococcus pneumoniae isolates

S. J. Salter, J. Hinds, K. A. Gould, L. Lambertsen, W. Hanage, M. Antonio, P. Turner, P. W M Hermans, H. J. Bootsma, Katherine L O'Brien, S. D. Bentley

Research output: Contribution to journalArticle

Abstract

The capsule polysaccharide locus (cps) is the site of the capsule biosynthesis gene cluster in encapsulated Streptococcus pneumoniae. A set of pneumococcal samples and nonpneumococcal streptococci from Denmark, the Gambia, the Netherlands, Thailand, the UK and the USA were sequenced at the cps locus to elucidate serologically mistyped or non-typable isolates. We identified a novel serotype 33B/33C mosaic capsule cluster and previously unseen serotype 22F capsule genes, disrupted and deleted cps clusters, the presence of aliB and nspA genes that are unrelated to capsule production, and similar genes in the non-pneumococcal samples. These data provide greater understanding of diversity at a locus which is crucial to the antigenic diversity of the pathogen and current vaccine strategies.

Original languageEnglish (US)
Pages (from-to)1560-1569
Number of pages10
JournalMicrobiology
Volume158
Issue number6
DOIs
StatePublished - Jun 2012

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Streptococcus pneumoniae
Capsules
Polysaccharides
Gambia
Genes
Antigenic Variation
Thailand
Denmark
Multigene Family
Streptococcus
Netherlands
Vaccines

ASJC Scopus subject areas

  • Microbiology

Cite this

Salter, S. J., Hinds, J., Gould, K. A., Lambertsen, L., Hanage, W., Antonio, M., ... Bentley, S. D. (2012). Variation at the capsule locus, cps, of mistyped and non-typable Streptococcus pneumoniae isolates. Microbiology, 158(6), 1560-1569. https://doi.org/10.1099/mic.0.056580-0

Variation at the capsule locus, cps, of mistyped and non-typable Streptococcus pneumoniae isolates. / Salter, S. J.; Hinds, J.; Gould, K. A.; Lambertsen, L.; Hanage, W.; Antonio, M.; Turner, P.; Hermans, P. W M; Bootsma, H. J.; O'Brien, Katherine L; Bentley, S. D.

In: Microbiology, Vol. 158, No. 6, 06.2012, p. 1560-1569.

Research output: Contribution to journalArticle

Salter, SJ, Hinds, J, Gould, KA, Lambertsen, L, Hanage, W, Antonio, M, Turner, P, Hermans, PWM, Bootsma, HJ, O'Brien, KL & Bentley, SD 2012, 'Variation at the capsule locus, cps, of mistyped and non-typable Streptococcus pneumoniae isolates', Microbiology, vol. 158, no. 6, pp. 1560-1569. https://doi.org/10.1099/mic.0.056580-0
Salter SJ, Hinds J, Gould KA, Lambertsen L, Hanage W, Antonio M et al. Variation at the capsule locus, cps, of mistyped and non-typable Streptococcus pneumoniae isolates. Microbiology. 2012 Jun;158(6):1560-1569. https://doi.org/10.1099/mic.0.056580-0
Salter, S. J. ; Hinds, J. ; Gould, K. A. ; Lambertsen, L. ; Hanage, W. ; Antonio, M. ; Turner, P. ; Hermans, P. W M ; Bootsma, H. J. ; O'Brien, Katherine L ; Bentley, S. D. / Variation at the capsule locus, cps, of mistyped and non-typable Streptococcus pneumoniae isolates. In: Microbiology. 2012 ; Vol. 158, No. 6. pp. 1560-1569.
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