Variable Field Proton-Electron Double-Resonance Imaging: Application to pH mapping of aqueous samples

Valery V. Khramtsov, George L. Caia, Keerthi Shet, Eric Kesselring, Sergey Petryakov, Jay L. Zweier, Alexandre Samouilov

Research output: Contribution to journalArticlepeer-review

Abstract

A new concept of Variable Field Proton-Electron Double-Resonance Imaging (VF PEDRI) is proposed. This allows for functional mapping using specifically designed paramagnetic probes (e.g. oxygen or pH mapping) with MRI high quality spatial resolution and short acquisition time. Studies performed at 200 G field MRI with phantoms show that a pH map of the sample can be extracted using only two PEDRI images acquired in 140 s at pre-selected EPR excitation fields providing pH resolution of 0.1 pH units and a spatial resolution of 1.25 mm. Note that while concept of functional VF PEDRI was demonstrated using the pH probe, it can be applied for studies of other biologically relevant parameters of the medium such as redox state, concentrations of oxygen or glutathione using specifically designed EPR probes.

Original languageEnglish (US)
Pages (from-to)267-273
Number of pages7
JournalJournal of Magnetic Resonance
Volume202
Issue number2
DOIs
StatePublished - Feb 2010
Externally publishedYes

Keywords

  • DNP
  • dynamic nuclear polarization
  • Functional imaging
  • Nitroxide
  • PEDRI
  • pH
  • Proton-Electron Double-Resonance Imaging
  • spin pH probe

ASJC Scopus subject areas

  • Nuclear and High Energy Physics
  • Biochemistry
  • Biophysics
  • Condensed Matter Physics

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