Variability in beta-globin and HPV DNA amplification by PCR from fixed tissues.

J. S. Park, J. F. Leake, B. K. Sharma, T. Toki, T. D. Kessis, R. A. Ambros, K. V. Shah

Research output: Contribution to journalArticle

Abstract

Paraffin-embedded tissues from a variety of sources and treated with different fixatives were tested for beta-globin and HPV amplification by polymerase chain reaction (PCR). In tests of tissues collected in the previous 2 to 3 yr, excellent rates (87% to 93%) for beta-globin amplification were obtained for specimens fixed in buffered formalin, Bouin's, and Hartmann's solutions. In contrast, the rate of beta-globin amplification was low for tissues fixed in Hollande's solution (7%) and in Hartmann's solution with eosin (33%). The results of beta-globin amplification from archival tissues stored for variable time periods showed no decrease in the amplification rate with longer periods of storage. Human papillomaviruses (HPVs) were identified in 17% of globin negative and in 43% of globin positive tissues. HPV-16 amplification was more efficient when the targeted DNA sequence was small. Variability in amplification depends not only on the type of fixative used, but also on other ill-defined factors. Therefore, conditions for optimal amplification should be determined before undertaking studies of archival material.

Original languageEnglish (US)
Pages (from-to)667-670
Number of pages4
JournalModern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc
Volume4
Issue number5
StatePublished - Sep 1991

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Fingerprint Dive into the research topics of 'Variability in beta-globin and HPV DNA amplification by PCR from fixed tissues.'. Together they form a unique fingerprint.

  • Cite this

    Park, J. S., Leake, J. F., Sharma, B. K., Toki, T., Kessis, T. D., Ambros, R. A., & Shah, K. V. (1991). Variability in beta-globin and HPV DNA amplification by PCR from fixed tissues. Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 4(5), 667-670.