VAMP-1, VAMP-2, and syntaxin-4 regulate ANP release from cardiac myocytes

Marcella Ferlito, William B. Fulton, Mohamed A. Zauher, Eduardo Marbán, Charles Steenbergen, Charles J. Lowenstein

Research output: Contribution to journalArticle

Abstract

ANP is a peptide released by cardiac myocytes that regulates blood pressure and natriuresis. However, the molecular mechanisms controlling ANP release from cardiac myocytes are not defined. We now identify three components of the exocytic machinery that regulate ANP release from atrial myocytes. We found that cardiac myocytes express N-ethylmaleimide sensitive factor (NSF), soluble NSF attachment protein (α-SNAP), and SNAP receptors (SNAREs). Additionally we found that specific SNARE molecules, VAMP-1 and VAMP-2, both co-sediment and co-localize with ANP. Also, one SNARE molecule, syntaxin-4, partially co-sediments and partially co-localizes with ANP. Furthermore, these three SNAREs, sytntaxin-4 and VAMP-1 and VAMP-2, form a SNARE complex inside cardiac myocytes. Finally, knockdown of VAMP-1, VAMP-2, or syntaxin-4 blocks regulated release of ANP. In contrast, silencing of VAMP-3 did not have an effect on ANP release. Our data suggest that three specific SNAREs regulate cardiac myocyte exocytosis of ANP. Pathways that modify the exocytic machinery may influence natriuresis and blood pressure.

Original languageEnglish (US)
Pages (from-to)791-800
Number of pages10
JournalJournal of Molecular and Cellular Cardiology
Volume49
Issue number5
DOIs
StatePublished - Nov 1 2010

Keywords

  • Exocytosis
  • Granule
  • Hypertension
  • N-ethylmaleimide sensitive factor
  • Nitric oxide
  • Secretion
  • Trafficking

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

Fingerprint Dive into the research topics of 'VAMP-1, VAMP-2, and syntaxin-4 regulate ANP release from cardiac myocytes'. Together they form a unique fingerprint.

  • Cite this