Validity testing of commercial urine cocaine metabolite assays

IV. Evaluation of the EMIT® d.a.u.® cocaine metabolite assay in a quantitative mode for detection of cocaine metabolite

E. J. Cone, D. Yousefnejad, S. L. Dickerson

Research output: Contribution to journalArticle

Abstract

The EMIT® d.a.u.® cocaine metabolite assay (EMIT dau) was evaluated in a quantitative mode for analysis of clinical specimens obtained after controlled cocaine administration to human subjects. The quantitative results showed high concordance with those of gas chromatography/mass spectrometry (GC/MS) assays of the same specimens for benzoylecgonine, and no false positive or false negative results were obtained. The evaluation also included analysis of standardized solution containing benzoylecgonine, cocaine, and other cocaine metabolites and isomers. The EMIT dau antibody demonstrated high selectivity for benzoylecgonine. The precision was somewhat less than that reported earlier for other commercial cocaine metabolite immunoassays. Quantitation of initial screening results from EMIT dau testing can serve as a useful guide for confirmation by GC/MS in forensic science urine testing.

Original languageEnglish (US)
Pages (from-to)786-791
Number of pages6
JournalJournal of Forensic Sciences
Volume35
Issue number4
StatePublished - 1990

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Cocaine
Urine
evaluation
Gas Chromatography-Mass Spectrometry
Enzyme Multiplied Immunoassay Technique
Forensic Sciences
science
Immunoassay
Antibodies
benzoylecgonine

Keywords

  • cocaine
  • EMIT®
  • toxicology

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Law

Cite this

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abstract = "The EMIT{\circledR} d.a.u.{\circledR} cocaine metabolite assay (EMIT dau) was evaluated in a quantitative mode for analysis of clinical specimens obtained after controlled cocaine administration to human subjects. The quantitative results showed high concordance with those of gas chromatography/mass spectrometry (GC/MS) assays of the same specimens for benzoylecgonine, and no false positive or false negative results were obtained. The evaluation also included analysis of standardized solution containing benzoylecgonine, cocaine, and other cocaine metabolites and isomers. The EMIT dau antibody demonstrated high selectivity for benzoylecgonine. The precision was somewhat less than that reported earlier for other commercial cocaine metabolite immunoassays. Quantitation of initial screening results from EMIT dau testing can serve as a useful guide for confirmation by GC/MS in forensic science urine testing.",
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AU - Yousefnejad, D.

AU - Dickerson, S. L.

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AB - The EMIT® d.a.u.® cocaine metabolite assay (EMIT dau) was evaluated in a quantitative mode for analysis of clinical specimens obtained after controlled cocaine administration to human subjects. The quantitative results showed high concordance with those of gas chromatography/mass spectrometry (GC/MS) assays of the same specimens for benzoylecgonine, and no false positive or false negative results were obtained. The evaluation also included analysis of standardized solution containing benzoylecgonine, cocaine, and other cocaine metabolites and isomers. The EMIT dau antibody demonstrated high selectivity for benzoylecgonine. The precision was somewhat less than that reported earlier for other commercial cocaine metabolite immunoassays. Quantitation of initial screening results from EMIT dau testing can serve as a useful guide for confirmation by GC/MS in forensic science urine testing.

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