Validity testing of commercial urine cocaine metabolite assays: III. Evaluation of an ezyme-linked immunosorbent assay (ELISA) for detection of cocaine and cocaine metabolite

E. J. Cone

Research output: Contribution to journalArticle

Abstract

A validity assessment study was performed on the Genetic Diagnostic Enzyme Immunoassay test kit, a new enzyme-linked immunosorbent assay (GDC ELISA) for detection of cocaine and cocaine metabolite in urine. A set of 290 urine specimens, comprised of clinical cocaine urines collected from 5 male subjects who had received single doses of intravenous cocaine, drug-free urines spiked with cocaine, cocaine metabolites, cocaine isomers, and other drug of abuse, were assayed by GDC ELISA. The results were compared with results by gas chromatography/mass spectrometry (GC/MS) assay for benzoylecgonine. Concordance was high between the GDC ELISA assay and GC/MS and with results reported earlier for other commercial assays. Detection times and specificity of the GDC ELISA antibody were most similar to those of the Abuscreen® radioimmunoassay for cocaine metabolite. Overall, the assay produced no false negative or false positive results and appeared to be a reliable screening test for detection of cocaine and benzoylecgonine in human urine.

Original languageEnglish (US)
Pages (from-to)991-995
Number of pages5
JournalJournal of Forensic Sciences
Volume34
Issue number4
StatePublished - 1989

Fingerprint

Immunosorbents
Cocaine
Urine
drug
evaluation
diagnostic
abuse
Gas Chromatography-Mass Spectrometry
Street Drugs
Immunoenzyme Techniques
Radioimmunoassay
Enzyme-Linked Immunosorbent Assay
time
Antibodies

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Law

Cite this

@article{fca4a3951451403091864c7bad645e90,
title = "Validity testing of commercial urine cocaine metabolite assays: III. Evaluation of an ezyme-linked immunosorbent assay (ELISA) for detection of cocaine and cocaine metabolite",
abstract = "A validity assessment study was performed on the Genetic Diagnostic Enzyme Immunoassay test kit, a new enzyme-linked immunosorbent assay (GDC ELISA) for detection of cocaine and cocaine metabolite in urine. A set of 290 urine specimens, comprised of clinical cocaine urines collected from 5 male subjects who had received single doses of intravenous cocaine, drug-free urines spiked with cocaine, cocaine metabolites, cocaine isomers, and other drug of abuse, were assayed by GDC ELISA. The results were compared with results by gas chromatography/mass spectrometry (GC/MS) assay for benzoylecgonine. Concordance was high between the GDC ELISA assay and GC/MS and with results reported earlier for other commercial assays. Detection times and specificity of the GDC ELISA antibody were most similar to those of the Abuscreen{\circledR} radioimmunoassay for cocaine metabolite. Overall, the assay produced no false negative or false positive results and appeared to be a reliable screening test for detection of cocaine and benzoylecgonine in human urine.",
author = "Cone, {E. J.}",
year = "1989",
language = "English (US)",
volume = "34",
pages = "991--995",
journal = "Journal of Forensic Sciences",
issn = "0022-1198",
publisher = "Wiley-Blackwell",
number = "4",

}

TY - JOUR

T1 - Validity testing of commercial urine cocaine metabolite assays

T2 - III. Evaluation of an ezyme-linked immunosorbent assay (ELISA) for detection of cocaine and cocaine metabolite

AU - Cone, E. J.

PY - 1989

Y1 - 1989

N2 - A validity assessment study was performed on the Genetic Diagnostic Enzyme Immunoassay test kit, a new enzyme-linked immunosorbent assay (GDC ELISA) for detection of cocaine and cocaine metabolite in urine. A set of 290 urine specimens, comprised of clinical cocaine urines collected from 5 male subjects who had received single doses of intravenous cocaine, drug-free urines spiked with cocaine, cocaine metabolites, cocaine isomers, and other drug of abuse, were assayed by GDC ELISA. The results were compared with results by gas chromatography/mass spectrometry (GC/MS) assay for benzoylecgonine. Concordance was high between the GDC ELISA assay and GC/MS and with results reported earlier for other commercial assays. Detection times and specificity of the GDC ELISA antibody were most similar to those of the Abuscreen® radioimmunoassay for cocaine metabolite. Overall, the assay produced no false negative or false positive results and appeared to be a reliable screening test for detection of cocaine and benzoylecgonine in human urine.

AB - A validity assessment study was performed on the Genetic Diagnostic Enzyme Immunoassay test kit, a new enzyme-linked immunosorbent assay (GDC ELISA) for detection of cocaine and cocaine metabolite in urine. A set of 290 urine specimens, comprised of clinical cocaine urines collected from 5 male subjects who had received single doses of intravenous cocaine, drug-free urines spiked with cocaine, cocaine metabolites, cocaine isomers, and other drug of abuse, were assayed by GDC ELISA. The results were compared with results by gas chromatography/mass spectrometry (GC/MS) assay for benzoylecgonine. Concordance was high between the GDC ELISA assay and GC/MS and with results reported earlier for other commercial assays. Detection times and specificity of the GDC ELISA antibody were most similar to those of the Abuscreen® radioimmunoassay for cocaine metabolite. Overall, the assay produced no false negative or false positive results and appeared to be a reliable screening test for detection of cocaine and benzoylecgonine in human urine.

UR - http://www.scopus.com/inward/record.url?scp=0024371528&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024371528&partnerID=8YFLogxK

M3 - Article

C2 - 2760594

AN - SCOPUS:0024371528

VL - 34

SP - 991

EP - 995

JO - Journal of Forensic Sciences

JF - Journal of Forensic Sciences

SN - 0022-1198

IS - 4

ER -