Validity testing of commercial urine cocaine metabolite assays: III. Evaluation of an ezyme-linked immunosorbent assay (ELISA) for detection of cocaine and cocaine metabolite

E. J. Cone

Research output: Contribution to journalArticle

Abstract

A validity assessment study was performed on the Genetic Diagnostic Enzyme Immunoassay test kit, a new enzyme-linked immunosorbent assay (GDC ELISA) for detection of cocaine and cocaine metabolite in urine. A set of 290 urine specimens, comprised of clinical cocaine urines collected from 5 male subjects who had received single doses of intravenous cocaine, drug-free urines spiked with cocaine, cocaine metabolites, cocaine isomers, and other drug of abuse, were assayed by GDC ELISA. The results were compared with results by gas chromatography/mass spectrometry (GC/MS) assay for benzoylecgonine. Concordance was high between the GDC ELISA assay and GC/MS and with results reported earlier for other commercial assays. Detection times and specificity of the GDC ELISA antibody were most similar to those of the Abuscreen® radioimmunoassay for cocaine metabolite. Overall, the assay produced no false negative or false positive results and appeared to be a reliable screening test for detection of cocaine and benzoylecgonine in human urine.

Original languageEnglish (US)
Pages (from-to)991-995
Number of pages5
JournalJournal of Forensic Sciences
Volume34
Issue number4
StatePublished - Jan 1 1989

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ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Genetics

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