Validation of the ALS assay in adult patients with culture confirmed pulmonary tuberculosis

Rokeya Sultana Rekha, S. M. Mostafa Kamal, Peter Andersen, Zeaur Rahim, Md Imranul Hoq, Gul Ara, Jan Andersson, David Sack, Rubhana Raqib

Research output: Contribution to journalArticle

Abstract

We have earlier shown that Bacille Calmette-Gue ́rin (BCG) vaccine-specific IgG Antibodies in Lymphocyte Supernatant (ALS) can be used for diagnosis of active tuberculosis (TB) in adults and children. Methodology/Principal Findings: The ALS method was validated in a larger cohort (n = 212) of patients with suspicion of pulmonary TB using multiple antigens (BCG, LAM, TB15.3, TB51A, CFP10-ESAT6-A, CFP, CW) from Mycobacterium tuberculosis. The sensitivity and specificity of the ALS assay was calculated using non-TB patients as controls. The sensitivity and the specificity were highest with BCG vaccine (90% and 88% respectively) followed by LAM (89% and 87% respectively). Simultaneous assessment of multiple antigen-specific antibodies increased sensitivity (91%) and specificity (88%). Using higher lymphocyte count in smaller volume of culture media increased detection and reduced the assay duration to ~30 hrs. Twenty one patients with clinical findings strongly suggestive of TB finally diagnosed as non-TB patients were positive by the ALS assay, of which 9 (43%) were positive for 7 antigens and 19 (90%) for at least 3 antigens. Conclusions/Significance: Our findings show that simultaneous detection of antigens improves the diagnostic potential of the ALS assay; the modified method increases sensitivity and can provide results in <48 hours, and enable detection of some cases of pulmonary TB that are not detectable by standard methods.

Original languageEnglish (US)
Article numbere16425
JournalPloS one
Volume6
Issue number1
DOIs
StatePublished - Feb 3 2011

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Rekha, R. S., Mostafa Kamal, S. M., Andersen, P., Rahim, Z., Imranul Hoq, M., Ara, G., Andersson, J., Sack, D., & Raqib, R. (2011). Validation of the ALS assay in adult patients with culture confirmed pulmonary tuberculosis. PloS one, 6(1), [e16425]. https://doi.org/10.1371/journal.pone.0016425