Validation of the ALS assay in adult patients with culture confirmed pulmonary tuberculosis

Rokeya Sultana Rekha, S. M. Mostafa Kamal, Peter Andersen, Zeaur Rahim, Md Imranul Hoq, Gul Ara, Jan Andersson, David Allen Sack, Rubhana Raqib

Research output: Contribution to journalArticle

Abstract

We have earlier shown that Bacille Calmette-Gue ́rin (BCG) vaccine-specific IgG Antibodies in Lymphocyte Supernatant (ALS) can be used for diagnosis of active tuberculosis (TB) in adults and children. Methodology/Principal Findings: The ALS method was validated in a larger cohort (n = 212) of patients with suspicion of pulmonary TB using multiple antigens (BCG, LAM, TB15.3, TB51A, CFP10-ESAT6-A, CFP, CW) from Mycobacterium tuberculosis. The sensitivity and specificity of the ALS assay was calculated using non-TB patients as controls. The sensitivity and the specificity were highest with BCG vaccine (90% and 88% respectively) followed by LAM (89% and 87% respectively). Simultaneous assessment of multiple antigen-specific antibodies increased sensitivity (91%) and specificity (88%). Using higher lymphocyte count in smaller volume of culture media increased detection and reduced the assay duration to ~30 hrs. Twenty one patients with clinical findings strongly suggestive of TB finally diagnosed as non-TB patients were positive by the ALS assay, of which 9 (43%) were positive for 7 antigens and 19 (90%) for at least 3 antigens. Conclusions/Significance: Our findings show that simultaneous detection of antigens improves the diagnostic potential of the ALS assay; the modified method increases sensitivity and can provide results in

Original languageEnglish (US)
Article numbere16425
JournalPLoS One
Volume6
Issue number1
DOIs
StatePublished - 2011

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Lymphocytes
Pulmonary Tuberculosis
Cell culture
tuberculosis
Assays
lymphocytes
lungs
antibodies
Antibodies
Antigens
antigens
assays
Sensitivity and Specificity
vaccines
Tuberculosis
Vaccines
antigen detection
lymphocyte count
Mycobacterium tuberculosis
Lymphocyte Count

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Rekha, R. S., Mostafa Kamal, S. M., Andersen, P., Rahim, Z., Imranul Hoq, M., Ara, G., ... Raqib, R. (2011). Validation of the ALS assay in adult patients with culture confirmed pulmonary tuberculosis. PLoS One, 6(1), [e16425]. https://doi.org/10.1371/journal.pone.0016425

Validation of the ALS assay in adult patients with culture confirmed pulmonary tuberculosis. / Rekha, Rokeya Sultana; Mostafa Kamal, S. M.; Andersen, Peter; Rahim, Zeaur; Imranul Hoq, Md; Ara, Gul; Andersson, Jan; Sack, David Allen; Raqib, Rubhana.

In: PLoS One, Vol. 6, No. 1, e16425, 2011.

Research output: Contribution to journalArticle

Rekha, RS, Mostafa Kamal, SM, Andersen, P, Rahim, Z, Imranul Hoq, M, Ara, G, Andersson, J, Sack, DA & Raqib, R 2011, 'Validation of the ALS assay in adult patients with culture confirmed pulmonary tuberculosis', PLoS One, vol. 6, no. 1, e16425. https://doi.org/10.1371/journal.pone.0016425
Rekha RS, Mostafa Kamal SM, Andersen P, Rahim Z, Imranul Hoq M, Ara G et al. Validation of the ALS assay in adult patients with culture confirmed pulmonary tuberculosis. PLoS One. 2011;6(1). e16425. https://doi.org/10.1371/journal.pone.0016425
Rekha, Rokeya Sultana ; Mostafa Kamal, S. M. ; Andersen, Peter ; Rahim, Zeaur ; Imranul Hoq, Md ; Ara, Gul ; Andersson, Jan ; Sack, David Allen ; Raqib, Rubhana. / Validation of the ALS assay in adult patients with culture confirmed pulmonary tuberculosis. In: PLoS One. 2011 ; Vol. 6, No. 1.
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