Validation of creatinine assays utilizing HPLC and IDMS traceable standards in sera of children

George J. Schwartz, Tai Kwong, Brian Erway, Bradley Warady, Lori J Sokoll, Stanley Hellerstein, Vikas Dharnidharka, Susan Furth, Alvaro Munoz

Research output: Contribution to journalArticle

Abstract

The purpose of this study was to validate serum creatinine (SCr) concentrations assayed in the Central Biochemistry Laboratory of the National Institutes of Health (NIH)-funded Chronic Kidney Disease in Children (CKiD) study utilizing an enzymatic assay (Siemens Advia 2400) against a method traceable to reference isotope dilution mass spectroscopy (IDMS) developed by the National Institute of Standards and Technology (NIST). High-performance liquid chromatography (HPLC) measured SCr after external validation utilizing IDMS-based standard reference materials. Sera from the first 201 subjects enrolled in CKiD were analyzed and compared for creatinine concentration by enzymatic and HPLC methods. Fifty "normal" pediatric sera were subsequently analyzed. Finally, a "pediatric" reference standard was prepared and examined for accuracy and precision. Enzymatic SCr concentrations (median 1.4 mg/dl) of CKiD subjects were well correlated with HPLC (r=0.984) but were slightly higher (+7%; p

Original languageEnglish (US)
Pages (from-to)113-119
Number of pages7
JournalPediatric Nephrology
Volume24
Issue number1
DOIs
StatePublished - 2009

Fingerprint

Isotopes
Mass Spectrometry
Creatinine
High Pressure Liquid Chromatography
Chronic Renal Insufficiency
Serum
Pediatrics
National Institutes of Health (U.S.)
Enzyme Assays
Biochemistry
Technology

Keywords

  • Enzymatic creatinine assay
  • Glomerular filtration rate
  • High-performance liquid chromatography
  • Isotope dilution mass spectroscopy
  • Jaffe creatinine assay
  • Pediatric standards

ASJC Scopus subject areas

  • Nephrology
  • Pediatrics, Perinatology, and Child Health

Cite this

Validation of creatinine assays utilizing HPLC and IDMS traceable standards in sera of children. / Schwartz, George J.; Kwong, Tai; Erway, Brian; Warady, Bradley; Sokoll, Lori J; Hellerstein, Stanley; Dharnidharka, Vikas; Furth, Susan; Munoz, Alvaro.

In: Pediatric Nephrology, Vol. 24, No. 1, 2009, p. 113-119.

Research output: Contribution to journalArticle

Schwartz, GJ, Kwong, T, Erway, B, Warady, B, Sokoll, LJ, Hellerstein, S, Dharnidharka, V, Furth, S & Munoz, A 2009, 'Validation of creatinine assays utilizing HPLC and IDMS traceable standards in sera of children', Pediatric Nephrology, vol. 24, no. 1, pp. 113-119. https://doi.org/10.1007/s00467-008-0957-0
Schwartz, George J. ; Kwong, Tai ; Erway, Brian ; Warady, Bradley ; Sokoll, Lori J ; Hellerstein, Stanley ; Dharnidharka, Vikas ; Furth, Susan ; Munoz, Alvaro. / Validation of creatinine assays utilizing HPLC and IDMS traceable standards in sera of children. In: Pediatric Nephrology. 2009 ; Vol. 24, No. 1. pp. 113-119.
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