Validation of a low cost computer-based method for quantification of immunohistochemistry-stained sections

Jill D. Montgomery, Heather R. Hensler, Lisa P. Jacobson, Frank J. Jenkins

Research output: Contribution to journalArticlepeer-review

Abstract

The aim of the present study was to determine if the Alpha DigiDoc RT system would be an effective method of quantifying immunohistochemical staining as compared with a manual counting method, which is considered the gold standard. Two readers were used to count 31 samples by both methods. The results obtained using the Bland-Altman for concordance deemed no statistical difference between the 2 methods. Thus, the Alpha DigiDoc RT system is an effective, low cost method to quantify immunohistochemical data.

Original languageEnglish (US)
Pages (from-to)400-404
Number of pages5
JournalApplied Immunohistochemistry and Molecular Morphology
Volume16
Issue number4
DOIs
StatePublished - Jul 2008

Keywords

  • IHC
  • Immunohistochemistry
  • Quantification

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Medical Laboratory Technology

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