Validation of a low cost computer-based method for quantification of immunohistochemistry-stained sections

Jill D. Montgomery, Heather R. Hensler, Lisa Paula Jacobson, Frank J. Jenkins

Research output: Contribution to journalArticle

Abstract

The aim of the present study was to determine if the Alpha DigiDoc RT system would be an effective method of quantifying immunohistochemical staining as compared with a manual counting method, which is considered the gold standard. Two readers were used to count 31 samples by both methods. The results obtained using the Bland-Altman for concordance deemed no statistical difference between the 2 methods. Thus, the Alpha DigiDoc RT system is an effective, low cost method to quantify immunohistochemical data.

Original languageEnglish (US)
Pages (from-to)400-404
Number of pages5
JournalApplied Immunohistochemistry and Molecular Morphology
Volume16
Issue number4
DOIs
StatePublished - Jul 2008

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Immunohistochemistry
Costs and Cost Analysis
Staining and Labeling

Keywords

  • IHC
  • Immunohistochemistry
  • Quantification

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Medical Laboratory Technology
  • Histology
  • Medicine(all)

Cite this

Validation of a low cost computer-based method for quantification of immunohistochemistry-stained sections. / Montgomery, Jill D.; Hensler, Heather R.; Jacobson, Lisa Paula; Jenkins, Frank J.

In: Applied Immunohistochemistry and Molecular Morphology, Vol. 16, No. 4, 07.2008, p. 400-404.

Research output: Contribution to journalArticle

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