Validation of a LC-APCI-MS/MS method for quantification of methadone, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) and 2-ethyl-5-methyl-3,3-diphenylpyraline (EMDP) in infant plasma following protein precipitation

Diaa M. Shakleya, Lauren M. Jansson, Marilyn A. Huestis

Research output: Contribution to journalArticle

Abstract

A validated, quantitative LC-APCI-MS/MS method for methadone, EDDP and EMDP in 200-μL plasma is presented. Specimen preparation was limited to protein precipitation and centrifugation. Chromatographic separation was achieved on a Synergi Hydro-RP 80A (50 mm × 2.0 mm, 4 μm) column with gradient elution. The assay was linear from 1 to 500 ng/mL, with intra- and inter-assay accuracy ≥87.5% and intra- and inter-assay precision <13.4% R.S.D. and recovery ≥87.5% for all analytes at 40 ng/mL. This analytical method is suitable for the accurate and precise determination of methadone and metabolites in human plasma specimens.

Original languageEnglish (US)
Pages (from-to)267-272
Number of pages6
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume856
Issue number1-2
DOIs
StatePublished - Sep 1 2007

Keywords

  • EDDP
  • EMDP
  • LC-APCI-MS/MS
  • Methadone
  • Plasma

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

Fingerprint Dive into the research topics of 'Validation of a LC-APCI-MS/MS method for quantification of methadone, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) and 2-ethyl-5-methyl-3,3-diphenylpyraline (EMDP) in infant plasma following protein precipitation'. Together they form a unique fingerprint.

  • Cite this