TY - GEN
T1 - Validation of a fiber-based confocal microscope for interventional image-guided procedures
T2 - Medical Imaging 2006: Physiology, Function, and Structure from Medical Images
AU - Herzka, Daniel
AU - Quijano, Jade
AU - Xie, Jianwu
AU - Krueger, Sascha
AU - Weiss, Steffen
AU - Abrat, Benjamin
AU - Osdoit, Anne
AU - Cavé, Charlotte
AU - Burnett, Christopher
AU - Danthi, S. Narasimhan
AU - Li, King
PY - 2006
Y1 - 2006
N2 - The concept of the biopsy is ubiquitous in current medical diagnosis of cancer and other diseases. The standard biopsy consists of removing a sample of tissue for evaluation and diagnosis, primarily to ascertain the presence of cancer cells by (histo)pathological analyses. However, the advent of new optical imaging modalities and targeted or "smart" agents, that have affinity for a select target, suggests the possibility of performing in vivo tissue characterization without the need for sample removal or the wait for histopathologic processing. Here we present work testing and validating a fiber-based confocal fluorescence microscopic imaging system intended for combination with a larger scale imaging modality (i.e. MRI or CT) to be used in image-guided in vivo tissue characterization. Fiber-based confocal fluorescence microscopic imaging experiments were performed (Cellvizio, Mauna Kea Technologies, Paris, France) in vivo in two mouse models including: 1) EGFP-expressing mouse melanoma model and 2) M21 mouse melanoma model. Both models are known to express integrin αvβ3, a cell-surface receptor protein. We also performed an experiment in ex vivo chicken muscle tissue labelled with a fluorescein isothiocyanate-lectin targeted compound. In the mouse models, contrast agents that targeted the integrin were injected and the contrast agent localization in tumor was verified by a whole-body multispectral imager. The fiber-based tool was sensitive enough to detect and image the tissue of interest in all different experiments, and was found appropriate for use in interventional catheter-based procedures.
AB - The concept of the biopsy is ubiquitous in current medical diagnosis of cancer and other diseases. The standard biopsy consists of removing a sample of tissue for evaluation and diagnosis, primarily to ascertain the presence of cancer cells by (histo)pathological analyses. However, the advent of new optical imaging modalities and targeted or "smart" agents, that have affinity for a select target, suggests the possibility of performing in vivo tissue characterization without the need for sample removal or the wait for histopathologic processing. Here we present work testing and validating a fiber-based confocal fluorescence microscopic imaging system intended for combination with a larger scale imaging modality (i.e. MRI or CT) to be used in image-guided in vivo tissue characterization. Fiber-based confocal fluorescence microscopic imaging experiments were performed (Cellvizio, Mauna Kea Technologies, Paris, France) in vivo in two mouse models including: 1) EGFP-expressing mouse melanoma model and 2) M21 mouse melanoma model. Both models are known to express integrin αvβ3, a cell-surface receptor protein. We also performed an experiment in ex vivo chicken muscle tissue labelled with a fluorescein isothiocyanate-lectin targeted compound. In the mouse models, contrast agents that targeted the integrin were injected and the contrast agent localization in tumor was verified by a whole-body multispectral imager. The fiber-based tool was sensitive enough to detect and image the tissue of interest in all different experiments, and was found appropriate for use in interventional catheter-based procedures.
KW - Image guided biopsy
KW - Interventional optical imaging
KW - Molecular imaging
KW - Optical confocal fluorescence microscopy
KW - Targeted optical imaging
UR - http://www.scopus.com/inward/record.url?scp=33745426058&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33745426058&partnerID=8YFLogxK
U2 - 10.1117/12.653272
DO - 10.1117/12.653272
M3 - Conference contribution
AN - SCOPUS:33745426058
SN - 0819461865
SN - 9780819461865
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Medical Imaging 2006
Y2 - 12 February 2006 through 14 February 2006
ER -