TY - JOUR
T1 - Validation and implementation of a liquid chromatography/tandem mass spectrometry assay to quantitate dimethyl benzoylphenylurea (BPU) and its five metabolites in human plasma and urine for clinical pharmacology studies
AU - Rudek, Michelle A.
AU - Zhao, Ming
AU - He, Ping
AU - Zabelina, Yelena
AU - Jin, Runyan
AU - Messersmith, Wells A.
AU - Wolff, Antonio C.
AU - Baker, Sharyn D.
N1 - Funding Information:
This work was supported by National Institutes of Health grants P30CA069773 and the Commonwealth Foundation for Cancer Research. We would like to thank Susan Davidson for quality assurance of the data contained in this manuscript.
PY - 2005/12/15
Y1 - 2005/12/15
N2 - A method has been developed for the quantitation of N-[4-(5-bromo-2- pyrimidinyloxy)-3-methylphenyl]-N′-(2-dimethylamino-benzoyl)urea (BPU) and its metabolites in human plasma and urine. BPU and metabolites were separated on a C18 column with acetonitrile-water mobile phase containing 0.1% formic acid using isocratic flow for 5 min. The analytes were monitored by tandem mass spectrometry. Calibration curves were generated over the range of 2.5-500 ng/mL for BPU, mmBPU, and aminoBPU in plasma; and 0.1-20, 0.1-20, 0.5-100, 10-2000, 1-200, and 3-600 ng/mL for BPU, mmBPU, aminoBPU, G280, G308, and G322 in urine, respectively. The method has been successfully applied to study the pharmacokinetics of BPU.
AB - A method has been developed for the quantitation of N-[4-(5-bromo-2- pyrimidinyloxy)-3-methylphenyl]-N′-(2-dimethylamino-benzoyl)urea (BPU) and its metabolites in human plasma and urine. BPU and metabolites were separated on a C18 column with acetonitrile-water mobile phase containing 0.1% formic acid using isocratic flow for 5 min. The analytes were monitored by tandem mass spectrometry. Calibration curves were generated over the range of 2.5-500 ng/mL for BPU, mmBPU, and aminoBPU in plasma; and 0.1-20, 0.1-20, 0.5-100, 10-2000, 1-200, and 3-600 ng/mL for BPU, mmBPU, aminoBPU, G280, G308, and G322 in urine, respectively. The method has been successfully applied to study the pharmacokinetics of BPU.
KW - Dimethyl benzoylphenylurea (BPU)
KW - LC/MS/MS
KW - Pharmacokinetics
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U2 - 10.1016/j.jchromb.2005.09.027
DO - 10.1016/j.jchromb.2005.09.027
M3 - Article
C2 - 16253576
AN - SCOPUS:28444450438
SN - 1570-0232
VL - 828
SP - 41
EP - 54
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
IS - 1-2
ER -