Validation and implementation of a liquid chromatography/tandem mass spectrometry assay to quantitate dimethyl benzoylphenylurea (BPU) and its five metabolites in human plasma and urine for clinical pharmacology studies

Michelle A. Rudek, Ming Zhao, Ping He, Yelena Zabelina, Runyan Jin, Wells A. Messersmith, Antonio C. Wolff, Sharyn D. Baker

Research output: Contribution to journalArticle

Abstract

A method has been developed for the quantitation of N-[4-(5-bromo-2- pyrimidinyloxy)-3-methylphenyl]-N′-(2-dimethylamino-benzoyl)urea (BPU) and its metabolites in human plasma and urine. BPU and metabolites were separated on a C18 column with acetonitrile-water mobile phase containing 0.1% formic acid using isocratic flow for 5 min. The analytes were monitored by tandem mass spectrometry. Calibration curves were generated over the range of 2.5-500 ng/mL for BPU, mmBPU, and aminoBPU in plasma; and 0.1-20, 0.1-20, 0.5-100, 10-2000, 1-200, and 3-600 ng/mL for BPU, mmBPU, aminoBPU, G280, G308, and G322 in urine, respectively. The method has been successfully applied to study the pharmacokinetics of BPU.

Original languageEnglish (US)
Pages (from-to)41-54
Number of pages14
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume828
Issue number1-2
DOIs
StatePublished - Dec 15 2005

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Keywords

  • Dimethyl benzoylphenylurea (BPU)
  • LC/MS/MS
  • Pharmacokinetics

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

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