Validation and Identification of Invasive Salmonella Serotypes in Sub-Saharan Africa by Multiplex Polymerase Chain Reaction

Hassan M. Al-Emran, Ralf Krumkamp, Denise Myriam Dekker, Daniel Eibach, Peter Aaby, Yaw Adu-Sarkodie, Mohammad Ali, Mathew P. Rubach, Morten Bjerregaard-Andersen, John A. Crump, Ligia Maria Cruz Espinoza, Sandra Valborg Løfberg, Amy Gassama Sow, Julian T. Hertz, Justin Im, Anna Jaeger, Leon Parfait Kabore, Frank Konings, Christian G. Meyer, Aissatou NiangGi Deok Pak, Ursula Panzner, Se Eun Park, Henintsoa Rabezanahary, Raphaël Rakotozandrindrainy, Tiana Mirana Raminosoa, Tsiriniaina Jean Luco Razafindrabe, Emmanuel Sampo, Heidi Schütt-Gerowitt, Nimako Sarpong, Abdramane Bassiahi Soura, Adama Tall, Vera Von Kalckreuth, Thomas F. Wierzba, Jürgen May, Florian Marks

Research output: Contribution to journalArticle

Abstract

Salmonella enterica serovar Typhi and nontyphoidal Salmonella (NTS) cause the majority of bloodstream infections in sub-Saharan Africa; however, serotyping is rarely performed. We validated a multiplex polymerase chain reaction (PCR) assay with the White-Kauffmann-Le Minor (WKLM) scheme of serotyping using 110 Salmonella isolates from blood cultures of febrile children in Ghana and applied the method in other Typhoid Fever Surveillance in Africa Program study sites. In Ghana, 47 (43%) S. Typhi, 36 (33%) Salmonella enterica serovar Typhimurium, 14 (13%) Salmonella enterica serovar Dublin, and 13 (12%) Salmonella enterica serovar Enteritidis were identified by both multiplex PCR and the WKLM scheme separately. Using the validated multiplex PCR assay, we identified 42 (66%) S. Typhi, 14 (22%) S. Typhimurium, 2 (3%) S. Dublin, 2 (3%) S. Enteritidis, and 4 (6%) other Salmonella species from the febrile patients in Burkina Faso, Guinea-Bissau, Madagascar, Senegal, and Tanzania. Application of this multiplex PCR assay in sub-Saharan Africa could advance the knowledge of serotype distribution of Salmonella.

Original languageEnglish (US)
Pages (from-to)s80-s82
JournalClinical Infectious Diseases
Volume62
DOIs
StatePublished - Mar 15 2016

Fingerprint

Africa South of the Sahara
Multiplex Polymerase Chain Reaction
Salmonella
Serotyping
Ghana
Salmonella enterica
Fever
Guinea-Bissau
Burkina Faso
Madagascar
Senegal
Salmonella enteritidis
Salmonella typhi
Tanzania
Typhoid Fever
Serogroup
Infection

Keywords

  • PCR
  • Salmonella spp
  • serotyping
  • sub-Saharan Africa

ASJC Scopus subject areas

  • Infectious Diseases
  • Microbiology (medical)

Cite this

Al-Emran, H. M., Krumkamp, R., Dekker, D. M., Eibach, D., Aaby, P., Adu-Sarkodie, Y., ... Marks, F. (2016). Validation and Identification of Invasive Salmonella Serotypes in Sub-Saharan Africa by Multiplex Polymerase Chain Reaction. Clinical Infectious Diseases, 62, s80-s82. https://doi.org/10.1093/cid/civ782

Validation and Identification of Invasive Salmonella Serotypes in Sub-Saharan Africa by Multiplex Polymerase Chain Reaction. / Al-Emran, Hassan M.; Krumkamp, Ralf; Dekker, Denise Myriam; Eibach, Daniel; Aaby, Peter; Adu-Sarkodie, Yaw; Ali, Mohammad; Rubach, Mathew P.; Bjerregaard-Andersen, Morten; Crump, John A.; Cruz Espinoza, Ligia Maria; Løfberg, Sandra Valborg; Gassama Sow, Amy; Hertz, Julian T.; Im, Justin; Jaeger, Anna; Kabore, Leon Parfait; Konings, Frank; Meyer, Christian G.; Niang, Aissatou; Pak, Gi Deok; Panzner, Ursula; Park, Se Eun; Rabezanahary, Henintsoa; Rakotozandrindrainy, Raphaël; Raminosoa, Tiana Mirana; Razafindrabe, Tsiriniaina Jean Luco; Sampo, Emmanuel; Schütt-Gerowitt, Heidi; Sarpong, Nimako; Soura, Abdramane Bassiahi; Tall, Adama; Von Kalckreuth, Vera; Wierzba, Thomas F.; May, Jürgen; Marks, Florian.

In: Clinical Infectious Diseases, Vol. 62, 15.03.2016, p. s80-s82.

Research output: Contribution to journalArticle

Al-Emran, HM, Krumkamp, R, Dekker, DM, Eibach, D, Aaby, P, Adu-Sarkodie, Y, Ali, M, Rubach, MP, Bjerregaard-Andersen, M, Crump, JA, Cruz Espinoza, LM, Løfberg, SV, Gassama Sow, A, Hertz, JT, Im, J, Jaeger, A, Kabore, LP, Konings, F, Meyer, CG, Niang, A, Pak, GD, Panzner, U, Park, SE, Rabezanahary, H, Rakotozandrindrainy, R, Raminosoa, TM, Razafindrabe, TJL, Sampo, E, Schütt-Gerowitt, H, Sarpong, N, Soura, AB, Tall, A, Von Kalckreuth, V, Wierzba, TF, May, J & Marks, F 2016, 'Validation and Identification of Invasive Salmonella Serotypes in Sub-Saharan Africa by Multiplex Polymerase Chain Reaction', Clinical Infectious Diseases, vol. 62, pp. s80-s82. https://doi.org/10.1093/cid/civ782
Al-Emran, Hassan M. ; Krumkamp, Ralf ; Dekker, Denise Myriam ; Eibach, Daniel ; Aaby, Peter ; Adu-Sarkodie, Yaw ; Ali, Mohammad ; Rubach, Mathew P. ; Bjerregaard-Andersen, Morten ; Crump, John A. ; Cruz Espinoza, Ligia Maria ; Løfberg, Sandra Valborg ; Gassama Sow, Amy ; Hertz, Julian T. ; Im, Justin ; Jaeger, Anna ; Kabore, Leon Parfait ; Konings, Frank ; Meyer, Christian G. ; Niang, Aissatou ; Pak, Gi Deok ; Panzner, Ursula ; Park, Se Eun ; Rabezanahary, Henintsoa ; Rakotozandrindrainy, Raphaël ; Raminosoa, Tiana Mirana ; Razafindrabe, Tsiriniaina Jean Luco ; Sampo, Emmanuel ; Schütt-Gerowitt, Heidi ; Sarpong, Nimako ; Soura, Abdramane Bassiahi ; Tall, Adama ; Von Kalckreuth, Vera ; Wierzba, Thomas F. ; May, Jürgen ; Marks, Florian. / Validation and Identification of Invasive Salmonella Serotypes in Sub-Saharan Africa by Multiplex Polymerase Chain Reaction. In: Clinical Infectious Diseases. 2016 ; Vol. 62. pp. s80-s82.
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AU - Dekker, Denise Myriam

AU - Eibach, Daniel

AU - Aaby, Peter

AU - Adu-Sarkodie, Yaw

AU - Ali, Mohammad

AU - Rubach, Mathew P.

AU - Bjerregaard-Andersen, Morten

AU - Crump, John A.

AU - Cruz Espinoza, Ligia Maria

AU - Løfberg, Sandra Valborg

AU - Gassama Sow, Amy

AU - Hertz, Julian T.

AU - Im, Justin

AU - Jaeger, Anna

AU - Kabore, Leon Parfait

AU - Konings, Frank

AU - Meyer, Christian G.

AU - Niang, Aissatou

AU - Pak, Gi Deok

AU - Panzner, Ursula

AU - Park, Se Eun

AU - Rabezanahary, Henintsoa

AU - Rakotozandrindrainy, Raphaël

AU - Raminosoa, Tiana Mirana

AU - Razafindrabe, Tsiriniaina Jean Luco

AU - Sampo, Emmanuel

AU - Schütt-Gerowitt, Heidi

AU - Sarpong, Nimako

AU - Soura, Abdramane Bassiahi

AU - Tall, Adama

AU - Von Kalckreuth, Vera

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