TY - JOUR
T1 - Validation and Identification of Invasive Salmonella Serotypes in Sub-Saharan Africa by Multiplex Polymerase Chain Reaction
AU - Al-Emran, Hassan M.
AU - Krumkamp, Ralf
AU - Dekker, Denise Myriam
AU - Eibach, Daniel
AU - Aaby, Peter
AU - Adu-Sarkodie, Yaw
AU - Ali, Mohammad
AU - Rubach, Mathew P.
AU - Bjerregaard-Andersen, Morten
AU - Crump, John A.
AU - Cruz Espinoza, Ligia Maria
AU - Løfberg, Sandra Valborg
AU - Gassama Sow, Amy
AU - Hertz, Julian T.
AU - Im, Justin
AU - Jaeger, Anna
AU - Kabore, Leon Parfait
AU - Konings, Frank
AU - Meyer, Christian G.
AU - Niang, Aissatou
AU - Pak, Gi Deok
AU - Panzner, Ursula
AU - Park, Se Eun
AU - Rabezanahary, Henintsoa
AU - Rakotozandrindrainy, Raphaël
AU - Raminosoa, Tiana Mirana
AU - Razafindrabe, Tsiriniaina Jean Luco
AU - Sampo, Emmanuel
AU - Schütt-Gerowitt, Heidi
AU - Sarpong, Nimako
AU - Soura, Abdramane Bassiahi
AU - Tall, Adama
AU - Von Kalckreuth, Vera
AU - Wierzba, Thomas F.
AU - May, Jürgen
AU - Marks, Florian
N1 - Publisher Copyright:
© 2016 The Author.
PY - 2016/3/15
Y1 - 2016/3/15
N2 - Salmonella enterica serovar Typhi and nontyphoidal Salmonella (NTS) cause the majority of bloodstream infections in sub-Saharan Africa; however, serotyping is rarely performed. We validated a multiplex polymerase chain reaction (PCR) assay with the White-Kauffmann-Le Minor (WKLM) scheme of serotyping using 110 Salmonella isolates from blood cultures of febrile children in Ghana and applied the method in other Typhoid Fever Surveillance in Africa Program study sites. In Ghana, 47 (43%) S. Typhi, 36 (33%) Salmonella enterica serovar Typhimurium, 14 (13%) Salmonella enterica serovar Dublin, and 13 (12%) Salmonella enterica serovar Enteritidis were identified by both multiplex PCR and the WKLM scheme separately. Using the validated multiplex PCR assay, we identified 42 (66%) S. Typhi, 14 (22%) S. Typhimurium, 2 (3%) S. Dublin, 2 (3%) S. Enteritidis, and 4 (6%) other Salmonella species from the febrile patients in Burkina Faso, Guinea-Bissau, Madagascar, Senegal, and Tanzania. Application of this multiplex PCR assay in sub-Saharan Africa could advance the knowledge of serotype distribution of Salmonella.
AB - Salmonella enterica serovar Typhi and nontyphoidal Salmonella (NTS) cause the majority of bloodstream infections in sub-Saharan Africa; however, serotyping is rarely performed. We validated a multiplex polymerase chain reaction (PCR) assay with the White-Kauffmann-Le Minor (WKLM) scheme of serotyping using 110 Salmonella isolates from blood cultures of febrile children in Ghana and applied the method in other Typhoid Fever Surveillance in Africa Program study sites. In Ghana, 47 (43%) S. Typhi, 36 (33%) Salmonella enterica serovar Typhimurium, 14 (13%) Salmonella enterica serovar Dublin, and 13 (12%) Salmonella enterica serovar Enteritidis were identified by both multiplex PCR and the WKLM scheme separately. Using the validated multiplex PCR assay, we identified 42 (66%) S. Typhi, 14 (22%) S. Typhimurium, 2 (3%) S. Dublin, 2 (3%) S. Enteritidis, and 4 (6%) other Salmonella species from the febrile patients in Burkina Faso, Guinea-Bissau, Madagascar, Senegal, and Tanzania. Application of this multiplex PCR assay in sub-Saharan Africa could advance the knowledge of serotype distribution of Salmonella.
KW - PCR
KW - Salmonella spp
KW - serotyping
KW - sub-Saharan Africa
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U2 - 10.1093/cid/civ782
DO - 10.1093/cid/civ782
M3 - Article
C2 - 26933026
AN - SCOPUS:84959915141
SN - 1058-4838
VL - 62
SP - s80-s82
JO - Clinical Infectious Diseases
JF - Clinical Infectious Diseases
ER -