Vacuolated Cytotrophoblast: A subpopulation of trophoblast in the chorion laeve

I. Tien Yeh, Dennis M. O'Connor, Robert J. Kurman

Research output: Contribution to journalArticle

Abstract

In summary, immunocytochemical analysis of the placenta and fetal membranes discloses two subpopulations of trophoblastic cells in the chorion laeve with distinct morphologic and immunocytochemical features. Both subpopulations are composed of mononucleate cells but one contains clear vacuolated cytoplasm whereas the other contains non-vacuolated eosinophilic cytoplasm. The vacuolated cells are placental alkaline phosphatase (PLAP) positive and human placental lactogen (hPL) negative, whereas the eosinophilic cells are PLAP negative and hPI, positive. Both cell types contained immunoreactive keratin and epithelial membrane antigen, but are negative for human chorionic gonadotropin(β), pregnancy specific beta i-glycoprotein, and prolactin. Electron microscopic studies of the vacuolated cells show that these cells contain numerous lucent non-membrane bound lipid droplets and pinocytotic vesicles. In addition they contain numerous intracellular filaments and desmosomes corresponding to the immunocytochemical localization of keratin. Their precise function is not clear, but the abundance of PLAP, an enzyme associated with absorption, suggests that these cells may be involved with maternal fetal transport. The vacuolated cells appear to be limited to the chorion. Their characteristic morphologic and immunocytochemical features and unique anatomic distribution suggests that they represent a distinctive subpopulation of trophoblastic cells for which we propose the term 'vacuolated cytotrophoblast'.

Original languageEnglish (US)
Pages (from-to)429-438
Number of pages10
JournalPlacenta
Volume10
Issue number5
DOIs
StatePublished - 1989

Fingerprint

Chorion
Trophoblasts
Keratins
Cytoplasm
Placental Lactogen
Extraembryonic Membranes
Mucin-1
Desmosomes
Chorionic Gonadotropin
Prolactin
Placenta
Glycoproteins
Mothers
Electrons

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Vacuolated Cytotrophoblast : A subpopulation of trophoblast in the chorion laeve. / Yeh, I. Tien; O'Connor, Dennis M.; Kurman, Robert J.

In: Placenta, Vol. 10, No. 5, 1989, p. 429-438.

Research output: Contribution to journalArticle

Yeh, I. Tien ; O'Connor, Dennis M. ; Kurman, Robert J. / Vacuolated Cytotrophoblast : A subpopulation of trophoblast in the chorion laeve. In: Placenta. 1989 ; Vol. 10, No. 5. pp. 429-438.
@article{f15c8404131a467eb3a3372c40042021,
title = "Vacuolated Cytotrophoblast: A subpopulation of trophoblast in the chorion laeve",
abstract = "In summary, immunocytochemical analysis of the placenta and fetal membranes discloses two subpopulations of trophoblastic cells in the chorion laeve with distinct morphologic and immunocytochemical features. Both subpopulations are composed of mononucleate cells but one contains clear vacuolated cytoplasm whereas the other contains non-vacuolated eosinophilic cytoplasm. The vacuolated cells are placental alkaline phosphatase (PLAP) positive and human placental lactogen (hPL) negative, whereas the eosinophilic cells are PLAP negative and hPI, positive. Both cell types contained immunoreactive keratin and epithelial membrane antigen, but are negative for human chorionic gonadotropin(β), pregnancy specific beta i-glycoprotein, and prolactin. Electron microscopic studies of the vacuolated cells show that these cells contain numerous lucent non-membrane bound lipid droplets and pinocytotic vesicles. In addition they contain numerous intracellular filaments and desmosomes corresponding to the immunocytochemical localization of keratin. Their precise function is not clear, but the abundance of PLAP, an enzyme associated with absorption, suggests that these cells may be involved with maternal fetal transport. The vacuolated cells appear to be limited to the chorion. Their characteristic morphologic and immunocytochemical features and unique anatomic distribution suggests that they represent a distinctive subpopulation of trophoblastic cells for which we propose the term 'vacuolated cytotrophoblast'.",
author = "Yeh, {I. Tien} and O'Connor, {Dennis M.} and Kurman, {Robert J.}",
year = "1989",
doi = "10.1016/0143-4004(89)90053-2",
language = "English (US)",
volume = "10",
pages = "429--438",
journal = "Placenta",
issn = "0143-4004",
publisher = "W.B. Saunders Ltd",
number = "5",

}

TY - JOUR

T1 - Vacuolated Cytotrophoblast

T2 - A subpopulation of trophoblast in the chorion laeve

AU - Yeh, I. Tien

AU - O'Connor, Dennis M.

AU - Kurman, Robert J.

PY - 1989

Y1 - 1989

N2 - In summary, immunocytochemical analysis of the placenta and fetal membranes discloses two subpopulations of trophoblastic cells in the chorion laeve with distinct morphologic and immunocytochemical features. Both subpopulations are composed of mononucleate cells but one contains clear vacuolated cytoplasm whereas the other contains non-vacuolated eosinophilic cytoplasm. The vacuolated cells are placental alkaline phosphatase (PLAP) positive and human placental lactogen (hPL) negative, whereas the eosinophilic cells are PLAP negative and hPI, positive. Both cell types contained immunoreactive keratin and epithelial membrane antigen, but are negative for human chorionic gonadotropin(β), pregnancy specific beta i-glycoprotein, and prolactin. Electron microscopic studies of the vacuolated cells show that these cells contain numerous lucent non-membrane bound lipid droplets and pinocytotic vesicles. In addition they contain numerous intracellular filaments and desmosomes corresponding to the immunocytochemical localization of keratin. Their precise function is not clear, but the abundance of PLAP, an enzyme associated with absorption, suggests that these cells may be involved with maternal fetal transport. The vacuolated cells appear to be limited to the chorion. Their characteristic morphologic and immunocytochemical features and unique anatomic distribution suggests that they represent a distinctive subpopulation of trophoblastic cells for which we propose the term 'vacuolated cytotrophoblast'.

AB - In summary, immunocytochemical analysis of the placenta and fetal membranes discloses two subpopulations of trophoblastic cells in the chorion laeve with distinct morphologic and immunocytochemical features. Both subpopulations are composed of mononucleate cells but one contains clear vacuolated cytoplasm whereas the other contains non-vacuolated eosinophilic cytoplasm. The vacuolated cells are placental alkaline phosphatase (PLAP) positive and human placental lactogen (hPL) negative, whereas the eosinophilic cells are PLAP negative and hPI, positive. Both cell types contained immunoreactive keratin and epithelial membrane antigen, but are negative for human chorionic gonadotropin(β), pregnancy specific beta i-glycoprotein, and prolactin. Electron microscopic studies of the vacuolated cells show that these cells contain numerous lucent non-membrane bound lipid droplets and pinocytotic vesicles. In addition they contain numerous intracellular filaments and desmosomes corresponding to the immunocytochemical localization of keratin. Their precise function is not clear, but the abundance of PLAP, an enzyme associated with absorption, suggests that these cells may be involved with maternal fetal transport. The vacuolated cells appear to be limited to the chorion. Their characteristic morphologic and immunocytochemical features and unique anatomic distribution suggests that they represent a distinctive subpopulation of trophoblastic cells for which we propose the term 'vacuolated cytotrophoblast'.

UR - http://www.scopus.com/inward/record.url?scp=0024456247&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024456247&partnerID=8YFLogxK

U2 - 10.1016/0143-4004(89)90053-2

DO - 10.1016/0143-4004(89)90053-2

M3 - Article

C2 - 2476802

AN - SCOPUS:0024456247

VL - 10

SP - 429

EP - 438

JO - Placenta

JF - Placenta

SN - 0143-4004

IS - 5

ER -