Vacuolar and plasma membrane stripping and autophagic elimination of Toxoplasma gondii in primed effector macrophages

Yun M. Ling, Michael H. Shaw, Carol Ayala, Isabelle Coppens, Gregory A. Taylor, David J.P. Ferguson, George S. Yap

Research output: Contribution to journalArticlepeer-review

Abstract

Apicomplexan protozoan pathogens avoid destruction and establish a replicative niche within host cells by forming a nonfusogenic parasitophorous vacuole (PV). Here we present evidence for lysosome-mediated degradation of Toxoplasma gondii after invasion of macrophages activated in vivo. Pathogen elimination was dependent on the interferon γ inducible-p47 GTPase, IGTP, required PI3K activity, and was preceded by PV membrane indentation, vesiculation, disruption, and, surprisingly, stripping of the parasite plasma membrane. Denuded parasites were enveloped in autophagosome-like vacuoles, which ultimately fused with lysosomes. These observations outline a series of mechanisms used by effector cells to redirect the fate of a classically nonfusogenic intracellular pathogen toward a path of immune elimination. JEM

Original languageEnglish (US)
Pages (from-to)2063-2071
Number of pages9
JournalJournal of Experimental Medicine
Volume203
Issue number9
DOIs
StatePublished - Sep 4 2006

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint

Dive into the research topics of 'Vacuolar and plasma membrane stripping and autophagic elimination of Toxoplasma gondii in primed effector macrophages'. Together they form a unique fingerprint.

Cite this