TY - JOUR
T1 - Vacuolar and plasma membrane stripping and autophagic elimination of Toxoplasma gondii in primed effector macrophages
AU - Ling, Yun M.
AU - Shaw, Michael H.
AU - Ayala, Carol
AU - Coppens, Isabelle
AU - Taylor, Gregory A.
AU - Ferguson, David J.P.
AU - Yap, George S.
PY - 2006/9/4
Y1 - 2006/9/4
N2 - Apicomplexan protozoan pathogens avoid destruction and establish a replicative niche within host cells by forming a nonfusogenic parasitophorous vacuole (PV). Here we present evidence for lysosome-mediated degradation of Toxoplasma gondii after invasion of macrophages activated in vivo. Pathogen elimination was dependent on the interferon γ inducible-p47 GTPase, IGTP, required PI3K activity, and was preceded by PV membrane indentation, vesiculation, disruption, and, surprisingly, stripping of the parasite plasma membrane. Denuded parasites were enveloped in autophagosome-like vacuoles, which ultimately fused with lysosomes. These observations outline a series of mechanisms used by effector cells to redirect the fate of a classically nonfusogenic intracellular pathogen toward a path of immune elimination. JEM
AB - Apicomplexan protozoan pathogens avoid destruction and establish a replicative niche within host cells by forming a nonfusogenic parasitophorous vacuole (PV). Here we present evidence for lysosome-mediated degradation of Toxoplasma gondii after invasion of macrophages activated in vivo. Pathogen elimination was dependent on the interferon γ inducible-p47 GTPase, IGTP, required PI3K activity, and was preceded by PV membrane indentation, vesiculation, disruption, and, surprisingly, stripping of the parasite plasma membrane. Denuded parasites were enveloped in autophagosome-like vacuoles, which ultimately fused with lysosomes. These observations outline a series of mechanisms used by effector cells to redirect the fate of a classically nonfusogenic intracellular pathogen toward a path of immune elimination. JEM
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U2 - 10.1084/jem.20061318
DO - 10.1084/jem.20061318
M3 - Article
C2 - 16940170
AN - SCOPUS:33748444233
SN - 0022-1007
VL - 203
SP - 2063
EP - 2071
JO - Journal of Experimental Medicine
JF - Journal of Experimental Medicine
IS - 9
ER -