TY - JOUR
T1 - Vaccination with live Plasmodium yoelii blood stage parasites under chloroquine cover induces cross-stage immunity against malaria liver stage
AU - Belnoue, Elodie
AU - Voza, Tatiana
AU - Costa, Fabio T.M.
AU - Grüner, Anne Charlotte
AU - Mauduit, Marjorie
AU - Rosa, Daniela Santoro
AU - Depinay, Nadya
AU - Kayibanda, Michèle
AU - Vigário, Ana Margarida
AU - Mazier, Dominique
AU - Snounou, Georges
AU - Sinnis, Photini
AU - Rénia, Laurent
PY - 2008/12/15
Y1 - 2008/12/15
N2 - Immunity to malaria has long been thought to be stage-specific. In this study we show that immunization of BALB/c mice with live erythrocytes infected with nonlethal strains of Plasmodium yoelii under curative chloroquine cover conferred protection not only against challenge by blood stage parasites but also against sporozoite challenge. This cross-stage protection was dosedependent and long lasting. CD4+ and CD8+ T cells inhibited malaria liver but not blood stage. Their effect was mediated partially by IFN-γ, and was completely dependent of NO. Abs against both pre-erythrocytic and blood parasites were elicited and were essential for protection against blood stage and liver stage parasites. Our results suggest that Ags shared by liver and blood stage parasites can be the foundation for a malaria vaccine that would provide effective protection against both pre-erythrocytic and erythrocytic asexual parasites found in the mammalian host.
AB - Immunity to malaria has long been thought to be stage-specific. In this study we show that immunization of BALB/c mice with live erythrocytes infected with nonlethal strains of Plasmodium yoelii under curative chloroquine cover conferred protection not only against challenge by blood stage parasites but also against sporozoite challenge. This cross-stage protection was dosedependent and long lasting. CD4+ and CD8+ T cells inhibited malaria liver but not blood stage. Their effect was mediated partially by IFN-γ, and was completely dependent of NO. Abs against both pre-erythrocytic and blood parasites were elicited and were essential for protection against blood stage and liver stage parasites. Our results suggest that Ags shared by liver and blood stage parasites can be the foundation for a malaria vaccine that would provide effective protection against both pre-erythrocytic and erythrocytic asexual parasites found in the mammalian host.
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U2 - 10.4049/jimmunol.181.12.8552
DO - 10.4049/jimmunol.181.12.8552
M3 - Article
C2 - 19050274
AN - SCOPUS:58849104986
SN - 0022-1767
VL - 181
SP - 8552
EP - 8558
JO - Journal of Immunology
JF - Journal of Immunology
IS - 12
ER -