UV irradiation induces a postreplication DNA damage checkpoint

A. John Callegari, Thomas J. Kelly

Research output: Contribution to journalArticle

Abstract

Eukaryotic cells irradiated with high doses of UV exhibit cell-cycle responses referred to as G1/S, intraS, and G2/M checkpoints. After a moderate UV dose that approximates sunlight exposure and is lethal to fission yeast checkpoint mutants, we found unexpectedly that these cell-cycle responses do not occur. Instead, cells at all stages of the cell cycle carry lesions into S phase and delay cell-cycle progression for hours after the completion of bulk DNA synthesis. Both DNA replication and the checkpoint kinase, Chk1, are required to generate this cell-cycle response. UV-irradiation of Δchk1 cells causes chromosome damage and loss of viability only after cells have replicated irradiated DNA and entered mitosis. These data suggest that an important physiological role of the cell-cycle response to UV is to provide time for postreplication repair.

Original languageEnglish (US)
Pages (from-to)15877-15882
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume103
Issue number43
DOIs
StatePublished - Oct 24 2006

Keywords

  • Cell cycle
  • Nucleotide excision repair
  • Yeast
  • chk1

ASJC Scopus subject areas

  • General

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