Utilization of Two-Photon FRET to Monitor SREBP Homodimer and Heterodimer Formation In Living Cells

Vickie J. LaMorte; Aikaterini Zoumi; Shrimati Datta; Cristen J. Wu; Timothy F. Osborne

doi:10.1117/12.478034

Utilization of Two-Photon FRET to Monitor SREBP Homodimer and Heterodimer Formation In Living Cells

Vickie J. LaMorte, Aikaterini Zoumi, Shrimati Datta, Cristen J. Wu, Timothy F. Osborne

Research output: Contribution to journal › Conference article › peer-review

Abstract

Key players in cholesterol regulation are the members of a family of transcription factors known as the Sterol Regulatory Binding Proteins or SREBPs. The cellular redundancy of these proteins is under investigation, and our findings suggest that where these proteins reside may provide evidence for differences in the molecular dynamics of their transcriptional activity. Specifically, we have found that GFP-tagged SREBP-2 in contrast to SREBP-1 resides in discrete nuclear foci. To further explore functional differences between SREBP-1 and SREBP-2 we have developed an approach to monitor hetero- and homodimer formation by two-photon imaging and spectroscopy of fluorescence resonance energy transfer (TPIS-FRET). TPIS-FRET results will be presented. Collectively, these findings support the possibility that differences in function between SREBP family members may be governed by their localization within the cell.

Original language	English (US)
Pages (from-to)	160-164
Number of pages	5
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	4963
DOIs	https://doi.org/10.1117/12.478034
State	Published - 2003
Externally published	Yes
Event	Multiphoton Microscopy in the Biomedical Sciences III - San Jose,CA, United States Duration: Jan 26 2003 → Jan 28 2003

Keywords

Fluorescence Resonance Energy Transfer (FRET)
GFP
Imaging
One-photon
Spectroscopy
Two-photon

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

Access to Document

10.1117/12.478034

Cite this

@article{f5ce04cf62b047279f7cf7ece2ff4184,

title = "Utilization of Two-Photon FRET to Monitor SREBP Homodimer and Heterodimer Formation In Living Cells",

abstract = "Key players in cholesterol regulation are the members of a family of transcription factors known as the Sterol Regulatory Binding Proteins or SREBPs. The cellular redundancy of these proteins is under investigation, and our findings suggest that where these proteins reside may provide evidence for differences in the molecular dynamics of their transcriptional activity. Specifically, we have found that GFP-tagged SREBP-2 in contrast to SREBP-1 resides in discrete nuclear foci. To further explore functional differences between SREBP-1 and SREBP-2 we have developed an approach to monitor hetero- and homodimer formation by two-photon imaging and spectroscopy of fluorescence resonance energy transfer (TPIS-FRET). TPIS-FRET results will be presented. Collectively, these findings support the possibility that differences in function between SREBP family members may be governed by their localization within the cell.",

keywords = "Fluorescence Resonance Energy Transfer (FRET), GFP, Imaging, One-photon, Spectroscopy, Two-photon",

author = "LaMorte, {Vickie J.} and Aikaterini Zoumi and Shrimati Datta and Wu, {Cristen J.} and Osborne, {Timothy F.}",

year = "2003",

doi = "10.1117/12.478034",

language = "English (US)",

volume = "4963",

pages = "160--164",

journal = "Proceedings of SPIE - The International Society for Optical Engineering",

issn = "0277-786X",

publisher = "SPIE",

note = "Multiphoton Microscopy in the Biomedical Sciences III ; Conference date: 26-01-2003 Through 28-01-2003",

}

TY - JOUR

T1 - Utilization of Two-Photon FRET to Monitor SREBP Homodimer and Heterodimer Formation In Living Cells

AU - LaMorte, Vickie J.

AU - Zoumi, Aikaterini

AU - Datta, Shrimati

AU - Wu, Cristen J.

AU - Osborne, Timothy F.

PY - 2003

Y1 - 2003

N2 - Key players in cholesterol regulation are the members of a family of transcription factors known as the Sterol Regulatory Binding Proteins or SREBPs. The cellular redundancy of these proteins is under investigation, and our findings suggest that where these proteins reside may provide evidence for differences in the molecular dynamics of their transcriptional activity. Specifically, we have found that GFP-tagged SREBP-2 in contrast to SREBP-1 resides in discrete nuclear foci. To further explore functional differences between SREBP-1 and SREBP-2 we have developed an approach to monitor hetero- and homodimer formation by two-photon imaging and spectroscopy of fluorescence resonance energy transfer (TPIS-FRET). TPIS-FRET results will be presented. Collectively, these findings support the possibility that differences in function between SREBP family members may be governed by their localization within the cell.

AB - Key players in cholesterol regulation are the members of a family of transcription factors known as the Sterol Regulatory Binding Proteins or SREBPs. The cellular redundancy of these proteins is under investigation, and our findings suggest that where these proteins reside may provide evidence for differences in the molecular dynamics of their transcriptional activity. Specifically, we have found that GFP-tagged SREBP-2 in contrast to SREBP-1 resides in discrete nuclear foci. To further explore functional differences between SREBP-1 and SREBP-2 we have developed an approach to monitor hetero- and homodimer formation by two-photon imaging and spectroscopy of fluorescence resonance energy transfer (TPIS-FRET). TPIS-FRET results will be presented. Collectively, these findings support the possibility that differences in function between SREBP family members may be governed by their localization within the cell.

KW - Fluorescence Resonance Energy Transfer (FRET)

KW - GFP

KW - Imaging

KW - One-photon

KW - Spectroscopy

KW - Two-photon

UR - http://www.scopus.com/inward/record.url?scp=0141605394&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0141605394&partnerID=8YFLogxK

U2 - 10.1117/12.478034

DO - 10.1117/12.478034

M3 - Conference article

AN - SCOPUS:0141605394

SN - 0277-786X

VL - 4963

SP - 160

EP - 164

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

T2 - Multiphoton Microscopy in the Biomedical Sciences III

Y2 - 26 January 2003 through 28 January 2003

ER -

Utilization of Two-Photon FRET to Monitor SREBP Homodimer and Heterodimer Formation In Living Cells

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this