Utilization of Two-Photon FRET to Monitor SREBP Homodimer and Heterodimer Formation In Living Cells

Vickie J. LaMorte, Aikaterini Zoumi, Shrimati Datta, Cristen J. Wu, Timothy F. Osborne

Research output: Contribution to journalConference articlepeer-review

Abstract

Key players in cholesterol regulation are the members of a family of transcription factors known as the Sterol Regulatory Binding Proteins or SREBPs. The cellular redundancy of these proteins is under investigation, and our findings suggest that where these proteins reside may provide evidence for differences in the molecular dynamics of their transcriptional activity. Specifically, we have found that GFP-tagged SREBP-2 in contrast to SREBP-1 resides in discrete nuclear foci. To further explore functional differences between SREBP-1 and SREBP-2 we have developed an approach to monitor hetero- and homodimer formation by two-photon imaging and spectroscopy of fluorescence resonance energy transfer (TPIS-FRET). TPIS-FRET results will be presented. Collectively, these findings support the possibility that differences in function between SREBP family members may be governed by their localization within the cell.

Original languageEnglish (US)
Pages (from-to)160-164
Number of pages5
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume4963
DOIs
StatePublished - Sep 29 2003
Externally publishedYes
EventMultiphoton Microscopy in the Biomedical Sciences III - San Jose,CA, United States
Duration: Jan 26 2003Jan 28 2003

Keywords

  • Fluorescence Resonance Energy Transfer (FRET)
  • GFP
  • Imaging
  • One-photon
  • Spectroscopy
  • Two-photon

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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