Utilization of HPASubC for the identification of sinusoid-specific proteins in the liver

Divine Favour Anene, Avi Z. Rosenberg, David E. Kleiner, Toby C Cornish, Marc K. Halushka

Research output: Contribution to journalArticlepeer-review

Abstract

Mass spectrometry-based proteomes of human organs and tissues are powerful tools but fail to capture protein localization and expression at the cellular level. For example, the proteome signal in liver represents the combined protein expression across diverse cellular constituents that include hepatocytes, Kupffer cells, endothelial cells, and others. We utilized HPASubC and the Human Protein Atlas (HPA) to identify the sinusoidal component of protein liver expression to further subset and organize this homogeneous signal. We evaluated 51-"109 liver images covering 13-"197 proteins from the HPA and discovered 1054 proteins that were exclusive to sinusoidal cells. Sinusoidal staining patterns were identified in a Kupffer cell (n = 247), endothelial cell (n = 358), or lymphocyte (n = 86) specific pattern. Two-hundred and thirty-nine of these proteins were not present in the NextProt or Human Proteome Map liver data sets, potentially expanding our knowledge of the liver proteome. We additionally demonstrate unique endothelial cell expression patterns that distinguish between portal vein, hepatic artery, capillary sinusoids, and central vein regions. These findings significantly improve our understanding of the liver proteome with insight into the endothelial complexity across the hepatic vascular network.

Original languageEnglish (US)
Pages (from-to)1623-1629
Number of pages7
JournalJournal of Proteome Research
Volume15
Issue number5
DOIs
StatePublished - May 6 2016

Keywords

  • endothelial cell
  • HPASubC
  • human protein atlas
  • Kupffer cell
  • liver
  • lymphocyte
  • proteome
  • tissue microarray

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

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