Utility of BRAF mutation detection in fine-needle aspiration biopsy samples read as "suspicious for papillary thyroid carcinoma"

Sebastian M. Jara, Ramneesh Bhatnagar, Hui Guan, Christopher D. Gocke, Syed Z. Ali, Ralph P. Tufano

Research output: Contribution to journalArticlepeer-review

Abstract

Background The purpose of this study was to evaluate the diagnostic utility of BRAF mutation testing on thyroid nodules "suspicious for papillary thyroid carcinoma" (PTC) cytology. Methods A chart review of patients with fine-needle aspiration (FNA) results "suspicious for PTC" with subsequent thyroidectomy was performed. Corresponding archived FNA slides underwent BRAF mutation testing. Results Sixty-six patients with FNA "suspicious for PTC" underwent thyroidectomy. Forty-two (63.6%) had PTC diagnosed on final histopathology, whereas 21 (31.8%) had benign findings. Thirty-five patients (83%) with histologically proven PTC underwent total thyroidectomy, whereas 7 (17%) underwent hemithyroidectomy. BRAF mutation was detected in 17 of 49 samples (34.6%) available for testing and had 45.5% sensitivity, 87.5% specificity, 88.2% positive predictive value (PPV), and 43.8% negative predictive value (NPV) for diagnosing PTC. Two of 4 patients (50%) who underwent hemithyroidectomy with subsequent completion thyroidectomy had mutated BRAF detected. Conclusion BRAF testing is a useful adjunct to improve PPV for patients with "suspicious for PTC" cytology.

Original languageEnglish (US)
Pages (from-to)1788-1793
Number of pages6
JournalHead and Neck
Volume37
Issue number12
DOIs
StatePublished - Dec 1 2015

Keywords

  • BRAF mutation
  • fine-needle aspiration biopsy
  • molecular marker testing
  • papillary thyroid carcinoma
  • suspicious for papillary carcinoma

ASJC Scopus subject areas

  • Otorhinolaryngology

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