Utility of BRAF mutation detection in fine-needle aspiration biopsy samples read as "suspicious for papillary thyroid carcinoma"

Sebastian M. Jara, Ramneesh Bhatnagar, Hui Guan, Christopher Gocke, Syed Z Ali, Ralph P Tufano

Research output: Contribution to journalArticle

Abstract

Background The purpose of this study was to evaluate the diagnostic utility of BRAF mutation testing on thyroid nodules "suspicious for papillary thyroid carcinoma" (PTC) cytology. Methods A chart review of patients with fine-needle aspiration (FNA) results "suspicious for PTC" with subsequent thyroidectomy was performed. Corresponding archived FNA slides underwent BRAF mutation testing. Results Sixty-six patients with FNA "suspicious for PTC" underwent thyroidectomy. Forty-two (63.6%) had PTC diagnosed on final histopathology, whereas 21 (31.8%) had benign findings. Thirty-five patients (83%) with histologically proven PTC underwent total thyroidectomy, whereas 7 (17%) underwent hemithyroidectomy. BRAF mutation was detected in 17 of 49 samples (34.6%) available for testing and had 45.5% sensitivity, 87.5% specificity, 88.2% positive predictive value (PPV), and 43.8% negative predictive value (NPV) for diagnosing PTC. Two of 4 patients (50%) who underwent hemithyroidectomy with subsequent completion thyroidectomy had mutated BRAF detected. Conclusion BRAF testing is a useful adjunct to improve PPV for patients with "suspicious for PTC" cytology.

Original languageEnglish (US)
Pages (from-to)1788-1793
Number of pages6
JournalHead and Neck
Volume37
Issue number12
DOIs
StatePublished - Dec 1 2015

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Fine Needle Biopsy
Thyroidectomy
Mutation
Cell Biology
Thyroid Nodule
Papillary Thyroid cancer
Sensitivity and Specificity

Keywords

  • BRAF mutation
  • fine-needle aspiration biopsy
  • molecular marker testing
  • papillary thyroid carcinoma
  • suspicious for papillary carcinoma

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Utility of BRAF mutation detection in fine-needle aspiration biopsy samples read as "suspicious for papillary thyroid carcinoma". / Jara, Sebastian M.; Bhatnagar, Ramneesh; Guan, Hui; Gocke, Christopher; Ali, Syed Z; Tufano, Ralph P.

In: Head and Neck, Vol. 37, No. 12, 01.12.2015, p. 1788-1793.

Research output: Contribution to journalArticle

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abstract = "Background The purpose of this study was to evaluate the diagnostic utility of BRAF mutation testing on thyroid nodules {"}suspicious for papillary thyroid carcinoma{"} (PTC) cytology. Methods A chart review of patients with fine-needle aspiration (FNA) results {"}suspicious for PTC{"} with subsequent thyroidectomy was performed. Corresponding archived FNA slides underwent BRAF mutation testing. Results Sixty-six patients with FNA {"}suspicious for PTC{"} underwent thyroidectomy. Forty-two (63.6{\%}) had PTC diagnosed on final histopathology, whereas 21 (31.8{\%}) had benign findings. Thirty-five patients (83{\%}) with histologically proven PTC underwent total thyroidectomy, whereas 7 (17{\%}) underwent hemithyroidectomy. BRAF mutation was detected in 17 of 49 samples (34.6{\%}) available for testing and had 45.5{\%} sensitivity, 87.5{\%} specificity, 88.2{\%} positive predictive value (PPV), and 43.8{\%} negative predictive value (NPV) for diagnosing PTC. Two of 4 patients (50{\%}) who underwent hemithyroidectomy with subsequent completion thyroidectomy had mutated BRAF detected. Conclusion BRAF testing is a useful adjunct to improve PPV for patients with {"}suspicious for PTC{"} cytology.",
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AB - Background The purpose of this study was to evaluate the diagnostic utility of BRAF mutation testing on thyroid nodules "suspicious for papillary thyroid carcinoma" (PTC) cytology. Methods A chart review of patients with fine-needle aspiration (FNA) results "suspicious for PTC" with subsequent thyroidectomy was performed. Corresponding archived FNA slides underwent BRAF mutation testing. Results Sixty-six patients with FNA "suspicious for PTC" underwent thyroidectomy. Forty-two (63.6%) had PTC diagnosed on final histopathology, whereas 21 (31.8%) had benign findings. Thirty-five patients (83%) with histologically proven PTC underwent total thyroidectomy, whereas 7 (17%) underwent hemithyroidectomy. BRAF mutation was detected in 17 of 49 samples (34.6%) available for testing and had 45.5% sensitivity, 87.5% specificity, 88.2% positive predictive value (PPV), and 43.8% negative predictive value (NPV) for diagnosing PTC. Two of 4 patients (50%) who underwent hemithyroidectomy with subsequent completion thyroidectomy had mutated BRAF detected. Conclusion BRAF testing is a useful adjunct to improve PPV for patients with "suspicious for PTC" cytology.

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