Utility of a commercial quantitative hepatitis C virus core antigen assay in a diagnostic laboratory setting

Koray Ergünay, Burçin Şener, Alpaslan Alp, Jale Karakaya, Güļen Hasçelik

Research output: Contribution to journalArticlepeer-review

Abstract

In this study, the utility and impact of hepatitis C virus (HCV) core antigen (Cag) detection via a commercial assay have been evaluated in diagnostic laboratory conditions. In a total of 272 samples from 226 individuals, HCV RNA was detected in 81.3% and anti-HCV antibody prevalence was 86.4%. HCV Cag reactivity was identified in 59.9% of the samples and in 75.8% with detectable RNA. The sensitivity and specificity of HCV Cag assay have been calculated as 75.8% and 95.1%, respectively, and agreement between HCV RNA and HCV Cag was moderate (κ = 0.554). HCV Cag and RNA levels were highly correlated (r = 0.915 and 0.937). A viral load threshold of 103 IU/mL has been recognized, above which the correlation with RNA became statistically significant and sensitivity increased to 90.9%. Detection and quantification of HCV core antigen have been observed as a strong alternative to nucleic acid testing for HCV monitorization.

Original languageEnglish (US)
Pages (from-to)486-491
Number of pages6
JournalDiagnostic Microbiology and Infectious Disease
Volume70
Issue number4
DOIs
StatePublished - Aug 2011

Keywords

  • Assay
  • Core antigen
  • HCV
  • Hepatitis C virus
  • Quantitative

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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