Uterine vein prostaglandin levels in late pregnant dogs

John G. Gerber, Walter C. Hubbard, Alan S. Nies

Research output: Contribution to journalArticlepeer-review

Abstract

We studied the uterine venous plasma concentrations of prostaglandins E2, F, 15 keto 13,14 dihydro E2 and 15 keto 13,14 dihydro F in late pregnant dogs in order to evaluate the rates of production and metabolism of prostaglandin E2 and F in pregnancy in vivo. We used a very specific and sensitive gas chromatography-mass spectrometry assay to measure these prostaglandins. The uterine venous concentrations of prostaglandin E2 and 15 keto 13,14 dihydro E2 were 1.35±.27 ng/ml and 1.89±.37 ng/ml, respectively; however, we could not find any prostaglandin F and very little of its plasma metabolite in uterine venous plasma. Since uterine microsomes can generate prostaglandin F and E2 from endoperoxides, prostaglandin F production in vivo must be regulated through an enzymatic step after endoperoxide formation. Prostaglandin E2 is produced by pregnant canine uterus in quantities high enough to have a biological effect in late pregnancy; however, prostaglandin F does not appear to play a role at this stage of pregnancy.

Original languageEnglish (US)
Pages (from-to)623-627
Number of pages5
JournalProstaglandins
Volume17
Issue number4
DOIs
StatePublished - Apr 1979

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

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